Skip to main content
Addgene
Showing: 181 - 200 of 51330 results

  1. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid
    #101208
    Purpose
    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A and Q695A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101208-plasmid-map-sequence-id-198110

    Enlarge

  2. RP4-8

    Plasmid
    #79813
    Purpose
    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Gentamicin resistance gene
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. RP4-7

    Plasmid
    #79811
    Purpose
    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Chloramphenicol acetyltransferase
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. RP4-11

    Plasmid
    #79812
    Purpose
    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Kanamycin cassette and SacB gene (sacB )
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Km
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

    Enlarge

  7. pDGO143

    Plasmid
    #212042
    Purpose
    Clostridium thermocellum expression plasmid
    Depositor
    Lee Lynd
    Type
    Empty backbone
    Use
    Plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    212042-plasmid-map-sequence-id-426746

    Enlarge

  8. pAdDeltaF6

    Plasmid
    #112867
    Purpose
    AAV helper plasmid
    Depositor
    James M. Wilson
    Insert
    E4, E2a and VA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112867-plasmid-map-sequence-id-372973

    Enlarge

  9. pJC224

    Plasmid
    #231169
    Purpose
    Empty Module A plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231169-plasmid-map-sequence-id-460659

    Enlarge

  10. dSaCas9-KRAB

    Plasmid
    #205250
    Purpose
    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.
    Depositor
    Rasmus Bak
    Insert
    dSaCas9-KRAB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205250-plasmid-map-sequence-id-409107

    Enlarge

  11. pJC225

    Plasmid
    #231170
    Purpose
    Empty Module B plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231170-plasmid-map-sequence-id-459161

    Enlarge

  12. pJC227

    Plasmid
    #231172
    Purpose
    Empty Module C' plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids
    Depositor
    Daniel Voytas
    Type
    Empty backbone
    Use
    Subcloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231172-plasmid-map-sequence-id-459157

    Enlarge

  13. pAAV-Syn-iCre

    Plasmid
    #122518
    Purpose
    Cre expression
    Depositor
    Kimberly Ritola
    Insert
    iCre
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122518-plasmid-map-sequence-id-237797

    Enlarge

  14. pAAV-nEF Coff/Fon DREADD Gq-mCherry

    Plasmid
    #183534
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183534-plasmid-map-sequence-id-362029

    Enlarge

  15. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid
    #183532
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183532-plasmid-map-sequence-id-362037

    Enlarge

  16. pAAV-nEF Con/Foff DREADD Gq-mCherry

    Plasmid
    #183533
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183533-plasmid-map-sequence-id-362041

    Enlarge

  18. RP4-6

    Plasmid
    #80385
    Purpose
    Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Spectinomycin/Streptomycin
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Tags
    none
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. AV0.1-TBG-iCre

    Plasmid
    #192881
    Purpose
    expression of Cre
    Depositor
    Kazuhiro Oka
    Insert
    Cre
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192881-plasmid-map-sequence-id-378888

    Enlarge

  20. scAV0.1-TBG-iCre

    Plasmid
    #192878
    Purpose
    expression of Cre
    Depositor
    Kazuhiro Oka
    Insert
    Cre
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    192878-plasmid-map-sequence-id-378925

    Enlarge

Showing: 181 - 200 of 51330 results