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We narrowed to 54,169 results for: plasmid

Showing: 181 - 200 of 54169 results

  1. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  2. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid
    #101231
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101231-plasmid-map-sequence-id-197986

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  3. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid
    #101229
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101229-plasmid-map-sequence-id-197216

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  4. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  5. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid
    #101208
    Purpose
    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A and Q695A
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101208-plasmid-map-sequence-id-198110

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  6. RP4-8

    Plasmid
    #79813
    Purpose
    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Gentamicin resistance gene
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. RP4-7

    Plasmid
    #79811
    Purpose
    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Chloramphenicol acetyltransferase
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. RP4-11

    Plasmid
    #79812
    Purpose
    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Kanamycin cassette and SacB gene (sacB )
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Km
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

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  10. pAd5Rep2entire-ACGf

    Plasmid
    #237877
    Purpose
    AAV packaging plasmid: This platform plasmid provides adenoviral helper functions and the AAV2 rep gene.
    Depositor
    Florian Sonntag, Ascend Advanced Therapies
    Insert
    rep2 and E2A, E4, L4-22K/33K, VA RNA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237877-plasmid-map-sequence-id-475951

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  11. pAd5Rep2entire-ATGf

    Plasmid
    #237878
    Purpose
    AAV packaging plasmid: This platform plasmid provides adenoviral helper functions and the AAV2 rep gene.
    Depositor
    Florian Sonntag, Ascend Advanced Therapies
    Insert
    rep2 and E2A, E4, L4-22K/33K, VA RNA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237878-plasmid-map-sequence-id-475994

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  12. pMIHP-16228 C.FLAG.HA

    Plasmid
    #237566
    Purpose
    C.FLAG.HA Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, FLAG, and HA
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237566-plasmid-map-sequence-id-477166

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  13. pMIHP-16230 C.FLAG.NWS

    Plasmid
    #237567
    Purpose
    C.FLAG.NWS Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, FLAG, and NWS
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237567-plasmid-map-sequence-id-477153

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  14. pMIHP-16231 C.FLAG.OLLAS

    Plasmid
    #237568
    Purpose
    C.FLAG.OLLAS Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, FLAG, and OLLAS
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237568-plasmid-map-sequence-id-477165

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  15. pMIHP-16232 C.FLAG.S

    Plasmid
    #237569
    Purpose
    C.FLAG.S Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, FLAG, and S
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237569-plasmid-map-sequence-id-477164

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  16. pMIHP-12532 HAT.E.AU1

    Plasmid
    #237570
    Purpose
    HAT.E.AU1 Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    AU1, E, and HAT
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237570-plasmid-map-sequence-id-477137

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  17. pMIHP-12146 E.FLAG.C

    Plasmid
    #237572
    Purpose
    E.FLAG.C Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, E, and FLAG
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237572-plasmid-map-sequence-id-477170

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  18. pMIHP-12511 HAT.C.E

    Plasmid
    #237573
    Purpose
    HAT.C.E Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, E, and HAT
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237573-plasmid-map-sequence-id-477168

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  19. pMIHP-12647 S.C.E

    Plasmid
    #237574
    Purpose
    S.C.E Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    C, E, and S
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237574-plasmid-map-sequence-id-477169

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  20. pMIHP-12624 OLLAS.FLAG.E

    Plasmid
    #237575
    Purpose
    OLLAS.FLAG.E Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    E, FLAG, and OLLAS
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237575-plasmid-map-sequence-id-477185

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Showing: 181 - 200 of 54169 results