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We narrowed to 54,388 results for: plasmid

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Showing: 181 - 200 of 54388 results

  1. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid
    #101214
    Purpose
    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C and K855A
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101214-plasmid-map-sequence-id-197223

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  2. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid
    #101212
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    101212-plasmid-map-sequence-id-197040

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  3. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

    Plasmid
    #100716
    Purpose
    B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting ATP1A1 (cloned using BsmBI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available Since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100716-plasmid-map-sequence-id-193652

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  4. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  5. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid
    #101231
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101231-plasmid-map-sequence-id-197986

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  6. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid
    #101229
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101229-plasmid-map-sequence-id-197216

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  7. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  8. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid
    #101208
    Purpose
    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A and Q695A
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101208-plasmid-map-sequence-id-198110

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  9. RP4-8

    Plasmid
    #79813
    Purpose
    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Gentamicin resistance gene
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Promoter
    Own promoter
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. RP4-7

    Plasmid
    #79811
    Purpose
    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Chloramphenicol acetyltransferase
    Use
    Helper plasmid for mobilization
    Promoter
    Own promoter
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. RP4-11

    Plasmid
    #79812
    Purpose
    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Kanamycin cassette and SacB gene (sacB )
    Use
    Helper plasmid for mobilization
    Promoter
    Km
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pAd5Rep2entire-ACGf

    Plasmid
    #237877
    Purpose
    AAV packaging plasmid: This platform plasmid provides adenoviral helper functions and the AAV2 rep gene.
    Depositor
    Ascend Advanced Therapies, Florian Sonntag
    Insert
    rep2 and E2A, E4, L4-22K/33K, VA RNA
    Use
    AAV
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237877-plasmid-map-sequence-id-475951

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  15. pAd5Rep2entire-ATGf

    Plasmid
    #237878
    Purpose
    AAV packaging plasmid: This platform plasmid provides adenoviral helper functions and the AAV2 rep gene.
    Depositor
    Ascend Advanced Therapies, Florian Sonntag
    Insert
    rep2 and E2A, E4, L4-22K/33K, VA RNA
    Use
    AAV
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237878-plasmid-map-sequence-id-475994

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  16. pMIHP-12529 HAT.FLAG.VSVG

    Plasmid
    #241459
    Purpose
    HAT.FLAG.VSVG Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    FLAG, HAT, and VSVG
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    241459-plasmid-map-sequence-id-481836

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  17. pMIHP-12668 S.E.AU1

    Plasmid
    #241460
    Purpose
    S.E.AU1 Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    AU1, E, and S
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    241460-plasmid-map-sequence-id-481829

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  18. pMIHP-10519 E.HA.FLAG

    Plasmid
    #241462
    Purpose
    E.HA.FLAG Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    E, FLAG, and HA
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    241462-plasmid-map-sequence-id-481851

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  19. pMIHP-12524 HAT.FLAG.E

    Plasmid
    #241463
    Purpose
    HAT.FLAG.E Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    E, FLAG, and HAT
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    241463-plasmid-map-sequence-id-481830

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  20. pMIHP-12660 S.FLAG.E

    Plasmid
    #241464
    Purpose
    S.FLAG.E Flow Code Plasmid
    Depositor
    Adrian Liston
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    E, FLAG, and S
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    241464-plasmid-map-sequence-id-481858

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Showing: 181 - 200 of 54388 results