We narrowed to 7,480 results for: lif

1. pHAGE2 Lnp-mCherry

   Plasmid

   #86687

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   LNPK (LNPK Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF5B-FRT-AP-Sstr3-GFP-DEST

   Plasmid

   #49098

   Purpose

   Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Sstr3 (Sstr3 Mouse)
   

   Use

   Tags

   GFP and acceptor peptide (AP)

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX317-EGFP

   Plasmid

   #115439

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti_dCas9-KRAB-MeCP2

   Plasmid

   #122205

   Purpose

   Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Lentiviral

   Tags

   KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Cas9 (D10A, H840A)

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol1-UAS:CoChR-tdTomato

   Plasmid

   #124233

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   CoChR-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCCM’

   Plasmid

   #162709

   Purpose

   pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   p15A origin replaced with high-copy colE1 origin

   Promoter

   PLtet0-1 promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB’

   Plasmid

   #162703

   Purpose

   pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   pHnCB10 derived carboxysome operon, prk
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   5513T>G (tetR E37A); 7758G>T (second tet op…

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. RBD-Fc

    Plasmid

    #164221

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike RBD (S SARS-CoV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Sst44-GFP

    Plasmid

    #172310

    Purpose

    SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst44
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pB-Globin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ntn1-Fc-His

    Plasmid

    #72104

    Purpose

    Expresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn1 (Ntn1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR CD19 tandem CAR

    Plasmid

    #113019

    Purpose

    for lentivirus production of CD19 tandem CAR

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Mouse, Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-DsRed_IRES_EGFP

    Plasmid

    #92194

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol1-UAS:ChrimsonR-tdTomato

    Plasmid

    #124231

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChrimsonR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol1-UAS:GtACR2-tdTomato

    Plasmid

    #124236

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    GtACR2-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-VAPB

    Plasmid

    #108126

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:VAPB (VAPB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. N1-Tq-Ca-FLITS

    Plasmid

    #191454

    Purpose

    Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    Tq-Ca-FLITS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol1-UAS:ChR2(H134R)-tdTomato

    Plasmid

    #124237

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChR2(H134R)-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+ heiCas9

    Plasmid

    #186929

    Purpose

    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

    Depositor

    Joachim Wittbrodt

    Article

    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

    Insert

    myc-flexible linker-oNLS-Cas9-oNLS
    

    Use

    CRISPR

    Tags

    myc-flexible linker-optimizedNLS and optimizedNLS

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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