We narrowed to 44,084 results for: cha

1. Sc eRF3∆165N (WT)

   Plasmid

   #175418

   Purpose

   Encodes His-MBP-TEV-WT eRF3∆165N

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF3 (SUP35 Budding Yeast)
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   ∆165N

   Promoter

   T7

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV5-Flag-mEPLIN alpha

   Plasmid

   #40955

   Depositor

   Elizabeth Luna, Scott Maul

   Article

   Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

   Insert

   EPLIN alpha (Lima1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

3. LRP6 deltaN-pCS2-VSVG

   Plasmid

   #27281

   Depositor

   Xi He

   Article

   Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

   Insert

   low density lipoprotein receptor-related protein 6 (LRP6 Human)
   

   Use

   Xenopus injection

   Tags

   VSVG

   Expression

   Mutation

   contains amino acid residues 1370–1613 (extracell…

   Promoter

   Available Since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pV132-vCas9

   Plasmid

   #203223

   Purpose

   Mammalian expression of vCas9

   Depositor

   Phil Sharp, Robert Langer, Vikash Chauhan

   Article

   Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   vCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S55R R976A K1003A T1314R

   Promoter

   CBh

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDF-NtXNtS1R1R2B2

   Plasmid

   #160882

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcS-S1, rbcX, raf1, raf2, bsd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcS-S1: T7, chaperones: T7

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHL 246

   Plasmid

   #53014

   Purpose

   AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   fimS (off orientation)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pYZ164

   Plasmid

   #98408

   Purpose

   Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.his3 (his3 Fission Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. S3_001_026_MV2

   Plasmid

   #134422

   Purpose

   pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dCas9v2

   Plasmid

   #103140

   Purpose

   TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

   Depositor

   Jens Nielsen

   Article

   Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Removed scaffold gRNA from original plasmid

   Promoter

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L1_KanR-Ck1

    Plasmid

    #136123

    Purpose

    L1 in position 1 (next to LB in L2 construct), for KanR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:nptII_3t35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. yHS1

    Plasmid

    #159150

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. KBB364

    Plasmid

    #185083

    Purpose

    RFA1 fused to GFP under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NsvBa

    Plasmid

    #100015

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

    Depositor

    David Clapham, Paul DeCaen

    Article

    DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

    Insert

    NsvBa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UMG-SP-1_pET29b

    Plasmid

    #215433

    Purpose

    Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UMG-SP-2_pET29b

    Plasmid

    #215434

    Purpose

    Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TetO-FUW-mWnt1

    Plasmid

    #176486

    Purpose

    mouse Wnt1 cDNA in TetO backbone

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    mouse Wnt1 cDNA (Wnt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MBP-His6-TEV-MMS2

    Plasmid

    #25465

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    MMS2 (MMS2, UBC13 Budding Yeast)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-CIBN-CreC

    Plasmid

    #75267

    Purpose

    The first half of the photo-activatable cre recombinase

    Depositor

    Michael Bruchas

    Article

    Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

    Insert

    N-terminal portion of the CIB1 transcription factor
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mOtop3_pcDNA3

    Plasmid

    #114679

    Purpose

    This construct was used to express mouse Otop3 in HEK-293 cells

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 3 (Otop3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NylB_pET29b

    Plasmid

    #215414

    Purpose

    Expression construct for NylB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL3-VEGFR2-570

    Plasmid

    #21309

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pHL 1903

    Plasmid

    #53020

    Purpose

    AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    AAV, Asp terminator, and fimS

    Expression

    Bacterial

    Mutation

    Promoter

    fimS

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Use

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB9336 (pgRNA_II-1_NatMX)

    Plasmid

    #161589

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV5-Flag-mEPLIN beta

    Plasmid

    #40956

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

    Insert

    EPLIN beta (Lima1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pBAD-TRP120

    Plasmid

    #71732

    Purpose

    inducible expression of TRP120 in E. coli

    Depositor

    Jere McBride

    Article

    Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

    Insert

    TRP120
    

    Use

    Tags

    6xHIS and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pRG710_lexO-HO_LexA-TF_HIS3MX

    Plasmid

    #154819

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MXS_PGK::tTA2-bGHpA

    Plasmid

    #62448

    Purpose

    MXS_chaining vector with PGK::tTA2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. POM42-mCherry

    Plasmid

    #69856

    Purpose

    N-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCherry minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-NtC60αC20NtLC60β

    Plasmid

    #160877

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: T7, chaperones: T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. rtTA and TagBFP cassettes

    Plasmid

    #62452

    Purpose

    MXS_chaining vector with 2 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::NLS-TagBFPx3-bGHpA
    CMV::rtTA3-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-RaACR_687-EYFP

    Plasmid

    #111077

    Purpose

    Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RaACR_687
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MXS_PGK::rtTA3-bGHpA

    Plasmid

    #62446

    Purpose

    MXS_chaining vector with PGK::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSBL_DK187

    Plasmid

    #156423

    Purpose

    Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MXS_PGK::HygroR-bGHpA

    Plasmid

    #62436

    Purpose

    MXS_chaining vector with PGK::HygroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against hygromycin B with PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MXS_CMV::HygroR-bGHpA

    Plasmid

    #62435

    Purpose

    MXS_chaining vector with CMV::HygroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against hygromycin B with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJM126

    Plasmid

    #49339

    Purpose

    bacterial expression of RFA1, RFA2, and RFA3

    Depositor

    Steven Brill

    Article

    He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

    Inserts

    RFA3 (RFA3 Budding Yeast)
    RFA2 (RFA2 Budding Yeast)
    RFA1 (RFA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pKF-P14MM2AG5h

    Plasmid

    #128254

    Purpose

    Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3-optoB2-LYY-YFP

    Plasmid

    #75271

    Purpose

    G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    G-protein independent version of optoB2
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    point mutations at L72F,Y136G,Y224A

    Promoter

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. MXS_PGK::CreERT2-bGHpA

    Plasmid

    #62444

    Purpose

    MXS_chaining vector with PGK::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMG

    Plasmid

    #58991

    Purpose

    MSCV based expression vector - expresses GFP

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Type

    Empty backbone

    Use

    Retroviral; Mscv-based retroviral system that exp…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

44. MXS_CMV::CreERT2-bGHpA

    Plasmid

    #62443

    Purpose

    MXS_chaining vector with CMV::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. EmptyTranscriptionalReporter

    Plasmid

    #62453

    Purpose

    MXS_chaining vector with 2 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMVmin::NLS-Citrine-bGHpA
    CMV::NLS-Cherry-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pFUW-tetO-mOrange

    Plasmid

    #134298

    Purpose

    doxycycline-inducible expression of mouse mOrange in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    mOrange
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pOAD

    Plasmid

    #196825

    Purpose

    Yeast two-hybrid ; Gal4 activation domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 Activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pMH-macroHC

    Plasmid

    #82337

    Purpose

    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

    Depositor

    Michael Hothorn

    Article

    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

    Insert

    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    

    Use

    Tags

    6xHis non-cleavable and macro domain of human his…

    Expression

    Bacterial

    Mutation

    Truncated protein, amino acids 1-178

    Promoter

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCfB9337 (pgRNA_IV-1_NatMX)

    Plasmid

    #161590

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pILGFPU0 (R)

    Plasmid

    #83562

    Purpose

    Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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