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pBT195

Plasmid

#127843

Purpose

clone BE4max CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max without deaminase

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRibo-Sec-APEX2

Plasmid

#111699

Purpose

Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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PdarC1

Plasmid

#24835

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PdarC1

Tags

HA and Myc

Expression

Yeast

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A[T22N]

Plasmid

#24906

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pMV306-ribo-APEX2

Plasmid

#111700

Purpose

Theophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (integrating)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR143Bul

Plasmid

#21119

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-143 sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-P85A

Plasmid

#33062

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

P85A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-PM-BAF180-SH2

Plasmid

#41932

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-190

Plasmid

#99664

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain ( 125-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pOGG203

Plasmid

#115504

Purpose

Clone of a level 1 unit which encodes a constitutively-expressed mCherry

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUT1858-HQ858878

Plasmid

#27960

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bHLH transcription factor encoded by Os01g0928000 LOC_Os01g70310

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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sup35-C653R

Plasmid

#1198

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed cys 653 to arg

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
PrbC10

Plasmid

#24788

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pJZC35

Plasmid

#62325

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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His-myc-Med20 pBacPAK8

Plasmid

#15370

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

Med20

Use

Baculovirus construction

Tags

His and myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N33-170C

Plasmid

#17526

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 34-159 (paired domain)

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N215-273C

Plasmid

#17527

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 216-272 (homeodomain)

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 iota

Plasmid

#44937

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 iota (GRF12 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pUT1613-HQ858850

Plasmid

#27932

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bHLH transcription factor encoded by Os02g0433600 LOC_Os02g23823

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-cwo

Plasmid

#33155

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

Insert

cwo (cwo Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A[Q67L]

Plasmid

#24905

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pES001 (pQacA-deGFP)

Plasmid

#69030

Purpose

Reporter Plasmid - deGFP driven by a qacR responsive promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

deGFP

Expression

Bacterial

Promoter

qacA

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJZC102

Plasmid

#62337

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p6671 MSCV-IP N-HAonly 38E6

Plasmid

#44144

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV38E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
sup35-R320I

Plasmid

#1199

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed arg 320 to isoleucine

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
PCx35-pcDNA-S276A

Plasmid

#42924

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser276 to Ala

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1612-HQ858849

Plasmid

#27931

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bHLH transcription factor encoded by Os03g0135700 LOC_Os03g04310

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1750-HQ858873

Plasmid

#27955

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

WRKY transcription factor encoded by Os01g0714800 LOC_Os01g51690

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO DNAK

Plasmid

#19463

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

dnaK molecular chaperone DnaK

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pTOPO-rRNA-5‘ETS 347 to 631

Plasmid

#23994

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rRNA 5'ETS ntds 347 to 631

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pUT1180-HQ858802

Plasmid

#27884

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

C2C2-CO-like transcription factor encoded by Os06g0298200 LOC_Os06g19444

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B[T22N]

Plasmid

#24909

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8B

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 SSA1

Plasmid

#19516

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO V5 DNAK

Plasmid

#19517

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

dnaK molecular chaperone DnaK

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
PA1024_pET21a(+)C-tag

Plasmid

#14579

Depositor

SeWon Suh

Article

Ha et al J Biol Chem. 2006 Jul 7. 281(27):18660-7

Insert

2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pN1_NUP62-EGFP3

Plasmid

#252748

Purpose

Expression of NUP62 with three tandem EGFPs.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

E3xGFP

Expression

Mammalian

Available Since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pE-SUMO_PspCas13b

Plasmid

#252295

Purpose

Expresses PspCas13b (WT) in bacterial cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

PspCas13b (WT)

Expression

Bacterial

Mutation

WT

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV hUBC-stuffer-mCherry-P2A-PuroR

Plasmid

#253231

Purpose

Backbone for insert expression with mCherry-2A-PuroR from lentiviral vector with cloning sites

Depositor

Charles Gersbach

Article

Reisman et al bioRxiv 2025.10.11.681828

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pNORg11-mScarlet-I

Plasmid

#249341

Purpose

Mediterraneibacter gnavus - Escherichia coli shuttle vector with mScarlet-I under the control of the constitutive promoter

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Inserts

cymR
veg promoter
CuO
mScarlet-I

Use

E. coli - r. gnavus shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pNORg12-mScarlet-I

Plasmid

#249342

Purpose

Mediterraneibacter gnavus - Escherichia coli shuttle vector with mScarlet-I under the control of the cumate-inducible promoter

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Inserts

cymR
fdx promoter
veg promoter
CuO
mScarlet-I

Use

E. coli - r. gnavus shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pM2_PLLacO1_R1_mTagBFP2

Plasmid

#250898

Purpose

mTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mTagBFP2

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pM2_PBAD_R1_EGFP

Plasmid

#250900

Purpose

EGFP expressed under araBAD promoter; part of BNeu AS13; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PBAD

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplA W37V

Plasmid

#248802

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pM2_PLTetO1_R1_EGFP

Plasmid

#250899

Purpose

EGFP expressed under PL TetO1 promoter; part of BNeu AS12; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL TetO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pK1_PHL_R1_EGFP

Plasmid

#250888

Purpose

EGFP expressed under PHL synthetic promoter ; part of BNeu AS8; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHL

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge

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