Addgene

We narrowed to 13,587 results for: nts

Showing: 3001 - 3030 of 13587 results

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pSADdeltaG-GCaMP3-DsRedX

Plasmid

#32645

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GCaMP3
DsRedX

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
LI E-F nos-T c2

Plasmid

#54407

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nopaline synthase terminator

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pPSup2

Plasmid

#149418

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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LI B-C mOrange

Plasmid

#54427

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mOrange + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80004

Plasmid

#50325

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFH23

Plasmid

#128180

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH53

Plasmid

#128182

Purpose

Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010783

Plasmid

#46009

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010783 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pICSL80016

Plasmid

#50332

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, beta-glucuronidase (E. coli) with two introns

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C T-Saphire

Plasmid

#54424

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

T-saphhire + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFH58

Plasmid

#128184

Purpose

Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGR-ECK125108723

Plasmid

#46008

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK125108723 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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LI D-E T-Saph + linker c1

Plasmid

#54412

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + T-Sapphire

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICH72400

Plasmid

#50338

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFH24

Plasmid

#131763

Purpose

Level0 SpCas9, wheat codon optimized (version 2)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SpCas9, wheat codon optimized, version 2

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPV033

Plasmid

#178359

Purpose

Targeted gene knock-in plasmid for Schizophyllum commune

Depositor

Han Wösten

Article

Vonk et al Open Res Europe 2021, 1:140

Inserts

Left flank
nour_cassette
Right flank

Use

Fungal homologous recombination plasmid

Tags

Expression

Mutation

Promoter

S. commune gpd and n.a.

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LI B-C Cerulean

Plasmid

#54425

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP-Cerulean + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C YFP

Plasmid

#54423

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

YFP-Venus + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LII ins 2-3

Plasmid

#54379

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

insulator sequence, dummy 2-3

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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LII ins 4-5

Plasmid

#54380

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

insulator sequence, dummy 4-5

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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pFH92

Plasmid

#125820

Purpose

Level0 nScCas9-ABE7.10, wheat/human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nScCas9-ABE7.10, wheat/human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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p414TEF_Hsp82_CycTER

Plasmid

#61711

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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LI D-E YFP + linker c1

Plasmid

#54411

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + YFP Venus

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pPSdown2

Plasmid

#149419

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-RFP6

Plasmid

#106426

Purpose

H-RAN-RFP6 (RANbody RFP6::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-RFP6

Use

Tags

HA, His, and Multiple HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCF710

Plasmid

#121892

Purpose

EF1a-WNV-protease-mTagBFP2

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CTGA-R13-AKSRV

Plasmid

#63622

Purpose

CTGA-R13-AKSRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSRV RNAP

Use

Tags

Expression

Bacterial

Mutation

V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

Promoter

T5/Lac

Available since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGR-ECK120010800

Plasmid

#46010

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010800 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH52

Plasmid

#128181

Purpose

Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge