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Plasmid#236100PurposeEnhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.DepositorInsertLAMP1-ExRai-CKAR2 (LAMP1 Human, Synthetic)
UseTagsFull-length LAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4c/His-Xpress-HA-hBIG2(WT)
Plasmid#79432PurposeExpresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cellsDepositorInsertBIG2 (ARFGEF2 Human)
UseTagsHA, His6, and XpressExpressionMammalianMutationPromoterAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4c/His-Xpress-HA-hBIG1(WT)
Plasmid#79431PurposeExpresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cellsDepositorInsertBIG1 (ARFGEF1 Human)
UseTagsHA, His6, and XpressExpressionMammalianMutationC1758RPromoterAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Par6-ExRai-CKAR2
Plasmid#236101PurposeSubcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.DepositorInsertPar6C-ExRai-CKAR2 (PARD6A Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-CKAR2
Plasmid#236097PurposeCytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.DepositorInsertExRai-CKAR2-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP TFDP2
Plasmid#236445Purposetransient overexpression of TFDP2 in mammalian cellsDepositorInsertTFDP2 (TFDP2 Human)
UseTagsFLAG-FLAG-STREP-STREPExpressionMammalianMutationPromoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP TFDP1
Plasmid#236444Purposetransient overexpression of TFDP1 in mammalian cellsDepositorInsertTFDP1 (TFDP1 Human)
UseTagsFLAG-FLAG-STREP-STREPExpressionMammalianMutationPromoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Step-FLAG FBXO21
Plasmid#236458Purposetransient overexpression of FBXO21 in mammalian cellsDepositorInsertFBXO21 (FBXO21 Human)
UseTagsStrep-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP E2F6
Plasmid#236441Purposetransient overexpression of E2F6 in mammalian cellsDepositorInsertE2F6 (E2F6 Human)
UseTagsFLAG-FLAG-STREP-STREPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP E2F7
Plasmid#236442Purposetransient overexpression of E2F7 in mammalian cellsDepositorInsertE2F7 (E2F7 Human)
UseTagsFLAG-FLAG-STREP-STREPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP E2F4
Plasmid#236439Purposetransient overexpression of E2F4 in mammalian cellsDepositorInsertE2F4 (E2F4 Human)
UseTagsFLAG-FLAG-STREP-STREPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP E2F5
Plasmid#236440Purposetransient overexpression of E2F5 in mammalian cellsDepositorInsertE2F5 (E2F5 Human)
UseTagsFLAG-FLAG-STREP-STREPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Strep-FLAG KBP
Plasmid#236459Purposetransient overexpression of KBP in mammalian cellsDepositorInsertKBP (KIF1BP Human)
UseTagsStrep-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S-ectodomain
Plasmid#235984PurposeInternally tagged SARS-CoV-2 spike ecto-domainDepositorInsertSpike protein (S SARS-CoV-2)
UseTags8xHis and Twin-Strep tag and internal Twin-Strep …ExpressionMammalianMutationaa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…PromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike-HiBiT
Plasmid#191212PurposeTransient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assayDepositorInsertSARS-CoV-2 spike_WT (S SARS CoV-2)
UseTagsHiBiT (high affinity small nanoluciferase fragmen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT2-LaG16-3W
Plasmid#234425PurposeExpress APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linkerDepositorInsertAPT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT1-LaG16-3W
Plasmid#234424PurposeExpress APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linkerDepositorInsertAPT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT1
Plasmid#234423PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorInsertAPT1 fused to LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT2
Plasmid#234422PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorInsertAPT2 fused to LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT2-LaG16
Plasmid#234421PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorInsertAPT2 fused to LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT1-LaG16
Plasmid#234420PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorInsertAPT1 fused to LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 JDM37-GFPnb-mCh
Plasmid#235666PurposeExpresses designed activator of Hsp70 tagged with a GFP nano body and mCherryDepositorInsertJDM37-GFPnb-mCh (PMCH Human, Synthetic)
UseTagsGFP nanobody and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP/VSVG-rbPodxl
Plasmid#233260PurposeExpression of EGFP-tagged PODXL WTDepositorInsertPODXL (PODXL Rabbit)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DDR1-F866Y-V5/HIS
Plasmid#236009Purposeexpression of the F866Y kinase defective mutant variant of human DDR1 that might be associated with lung AdenocarcinomaDepositorInserthuman DDR1-F866Y receptor tyrosine kinase, full length (DDR1 Human)
UseTagsV5/HisExpressionMammalianMutationF866Y substitutionPromoterCMVAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 JDM16-GFPnb-mCh
Plasmid#235667PurposeExpresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherryDepositorInsertJDM16-GFPnb-mCh (Mch Synthetic)
UseTagsGFP nanobody and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-V561D-V5/HIS
Plasmid#234762PurposeExpression of the V561D mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor and GIST-Plus Syndrome, , constantly activatedDepositorInsertPDGFRA-V561D receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationV561D substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-P577S-V5/HIS
Plasmid#234760PurposeExpression of the P577S mutant variant of human PDGFRA, which might be associated with MelanomaDepositorInsertPDGFRA-P577S receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationP577S substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-R487L-V5/HIS
Plasmid#234761PurposeExpression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal TumorDepositorInsertPDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationR487L substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-D842V-V5/HIS
Plasmid#234763PurposeExpression of the D842V mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor, constantly activatedDepositorInsertPDGFRA-D842V receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationD842V substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-G853D-V5/HIS
Plasmid#234757PurposeExpression of the G853D mutant variant of human PDGFRA, which might be associated with MelanomaDepositorInsertPDGFRA-G853D receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationG853D substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-T674I-V5/HIS
Plasmid#234756PurposeExpression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinibDepositorInsertPDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationT674I substitutionPromoterCMVAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab11 Q70L in pcDNA3.1
Plasmid#90467PurposeRhoA mediated cell migrationDepositorInsertRab11 Q70L
UseTagsmEYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP
Plasmid#233356PurposeCMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_off-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233353PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP
Plasmid#233354PurposeCMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP
Plasmid#233359PurposeCMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP
Plasmid#233360PurposeCMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP
Plasmid#233358PurposeCMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP
Plasmid#233355PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_on-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP
Plasmid#233362PurposeCMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP
Plasmid#233361PurposeCMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD
Plasmid#233363PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubuleDepositorInsertKinprola_PKA-mEGFP-MTBD
UseTagsmEGFP-MTBDExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP
Plasmid#233357PurposeCMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-K627M-V5/HIS
Plasmid#234755PurposeExpression of the K627M kinase defective mutant variant of human PDGFRADepositorInsertPDGFRA-K627M receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationK627M substitutionPromoterCMVAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3
Plasmid#233062PurposeExpresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.DepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsAlfa and HiBiTExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt
Plasmid#233240PurposeParallel coiled-coil with (GGS)3 linker to prone condensationDepositorInsertVh-clover-GCN4-(GGS)3-Vt
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt
Plasmid#233241PurposeParallel coiled-coil with (GGS)12 linker to prevent condensationDepositorInsertVh-clover-GCN4-(GGS)12-Vt
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FFSS-BACH1 C122E
Plasmid#232274PurposeExpression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell linesDepositorInsertBACH1 (BACH1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationchanged cysteine 122 to glutamic acidPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO T21R-T2A-mCherry
Plasmid#225585PurposeMammalian expression vector for Doxycycline inducible expression of TRIM21 RING with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1-N2 (1-399)
Plasmid#225452PurposeExpress mEGFP-fusion protein of FXR1 fragment (1-399)DepositorInsertFXR1 (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationN2 fragment (1-399)PromoterCMVAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only