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Plasmid#29395DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationY410F mutant gene including genomic sequence 1432…PromoterAvailable sinceMay 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBF-E6
Plasmid#21696DepositorInsertprotein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
UseTagsProtein G B1 domainExpressionBacterialMutationGB1 (1-56aa) fusion tag with mutations at three p…PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf217
Plasmid#12905PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf217
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf219
Plasmid#12907PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf219
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-sfGFP (TB40/E)
Plasmid#136451PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti 4.1 Ex miR200c-141
Plasmid#35534Purpose3rd generation lentiviral vectorDepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-recA56
Plasmid#89962PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseTagsExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTet and proDAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3
Plasmid#21073PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFPExpressionMammalianMutationFirst ATG is not included.PromoterCMVAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralTagsExpressionMutationWTPromoterU6Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR1-optoDroplet
Plasmid#111509PurposeFor optogenetic clustering of FGFR1DepositorInsertFGFR1 (FGFR1 Human)
UseLentiviralTagsCry2, FUS, Fusion Red, and Myristoylation sequenc…ExpressionMutationPromoterSFFVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MYR-FR-C2D-EGFR
Plasmid#179262PurposeLight-induced clustering of EGFR cytosolic domainsDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsCry2 PHR, FUS, FusionRed, and Myristoylation sequ…ExpressionMutationPromoterSFFVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBtet rtTA G72V SE Δsplice GP Luc
Plasmid#194328PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightnessDepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationTet-responsive promotor related mutations: G72V i…PromoterTight tet-responsive promotorAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-BC0491-luc2
Plasmid#227133PurposeBarcoded assay, cAMP/Ca2+ sensor; barcode BC0491DepositorInsert6x clustered CRE element linked to CMV minimal promoter driving barcode 0491 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLuc
Plasmid#83281Purposerecombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)DepositorInsertFirefly Luciferase
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HyPer-DAAO-NES
Plasmid#119164PurposeFusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.DepositorInsertHyPer-DAAO-NES
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)dTomato
Plasmid#200113PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence, no other pr…Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-spTorA-GFP-H6C
Plasmid#168517PurposeGFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.DepositorInsertspTorA-GFP-H6C
UseTags6xHisC tag and spTorA (signal peptide of E. coli …ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax
Plasmid#190153PurposeExpresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only AA #1-94PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E)dTomato
Plasmid#200110PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element, no other promoter elemen…Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only