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We narrowed to 57,299 results for: plasmid

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Showing: 2001 - 2020 of 57299 results

  1. pX458M-53BP1-DN1S

    Plasmid
    #131045
    Purpose
    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.
    Depositor
    Punam Malik
    Publication
    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:
    Insert
    SpCas9-53BP1-DN1S (TP53BP1 Human)
    Use
    CRISPR
    Tags
    GFP and SpCas9
    Expression
    Mammalian
    Mutation
    Only expressing residues 1231-1644 of complete 53…
    Promoter
    CBH
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    131045-plasmid-map-sequence-id-258265

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  5. CDH5-AbR-GFP

    Plasmid
    #122970
    Purpose
    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.
    Depositor
    Budd A. Tucker
    Inserts
    CDH5p
    CopGFP
    Zeocin
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    NA
    Available since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    122970-plasmid-map-sequence-id-244602

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  6. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162667-plasmid-map-sequence-id-321443

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  7. hSynapsin-PE2-Cterminal-lenti

    Plasmid
    #135956
    Purpose
    Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) C-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    MMLV RT(D200N, T306K, W313F, T330P, L603W)
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135956-plasmid-map-sequence-id-269513

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  11. pJBEI-6409

    Plasmid
    #47048
    Purpose
    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Publication
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Mutation
    R364S mutation in MK
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    47048-plasmid-map-sequence-id-496831

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  13. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid
    #170186
    Purpose
    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing
    Depositor
    Bradley Bernstein
    Publication
    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:
    Insert
    CreERT2-T2A-eBFP2
    Use
    Lentiviral
    Promoter
    EF1 alpha
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    170186-plasmid-map-sequence-id-336799

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  16. TRMPVIR

    Plasmid
    #27994
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Publication
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TRMPVIR (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    27994-plasmid-map-sequence-id-13249

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  18. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Insert
    centrin-1 (CETN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    72641-plasmid-map-sequence-id-132790

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  19. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    185912-plasmid-map-sequence-id-367362

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Showing: 2001 - 2020 of 57299 results