We narrowed to 5,023 results for: cre encoding plasmid

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pDMJ2-SARS-CoV-2-Spike (Beta)

Plasmid

#241109

Purpose

Expresses SARS-CoV-2 spike (Beta) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Beta) (S SARS-CoV-2)

Expression

Mammalian

Mutation

L18F,D80A,D215G,L241del,L242del,A243del,K417N,E48…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Alpha)

Plasmid

#241032

Purpose

Expresses SARS-CoV-2 spike (Alpha) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Alpha) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69del,V70del,Y144del,N501Y,A570D,D614G,P681H,T71…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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VPX-mRNA

Plasmid

#227679

Purpose

plasmid for VPX mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

VPX (HIV2gp3 )

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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PEmax_2star_pet21A

Plasmid

#227675

Purpose

Expression plasmid for PEMAX** protein in bacteria.

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

PEmax_2_star

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-1

Plasmid

#83934

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-BNT162b2ΔUTR

Plasmid

#171214

Purpose

Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

Depositor

Liang Wei Wang

Article

Low et al medRxiv 2021.04.27.21256151

Insert

BNT162b2ΔUTR

Use

Gateway cloning

Mutation

Kozak sequence changed from CCCGCCACC to GGCTGCAC…

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PP1Cbeta

Plasmid

#31677

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf35

Plasmid

#12723

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf35 (J3R Vaccinia Virus)

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pLenti-sgCTRL-2

Plasmid

#83935

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-3

Plasmid

#83936

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-BCL2 3'UTR-mir200c-mut

Plasmid

#84598

Purpose

Renilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

BCL2 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on BCL2 3'…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCIneoRL-TOB1 3'UTR-mir200c-mut

Plasmid

#84590

Purpose

Renilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

TOB1 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on TOB1 3'…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf200

Plasmid

#12888

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf200

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pETM6-G6-vioABE-4A6-vioC-3A2-vioD

Plasmid

#73440

Purpose

Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE-P4A6-vioC-P3A2-vioD

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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eeBxb1_IVT

Plasmid

#222342

Purpose

Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Template for in vitro transcription

Promoter

T7 (inactivated)

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1b-ST-WPRE

Plasmid

#214967

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1b-ST

Use

AAV

Promoter

human Synapsin

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1s-ST-WPRE

Plasmid

#214968

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1s-ST

Use

AAV

Promoter

human Synapsin

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pECE-M2-BAIAP2 wt

Plasmid

#31656

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

Plasmid

#21850

Depositor

David Ron

Article

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

ATF4 (Atf4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

ATF4 5' UTR and AUG fused to Luciferase driv…

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-sgALCAM

Plasmid

#83929

Purpose

Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgALCAM

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

Plasmid

#239608

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.18 NR0B1-Luc

Plasmid

#100983

Purpose

NR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoter

Depositor

Lee Helman

Article

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

NR0B1 (NR0B1 Human)

Expression

Mammalian

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-EEA1

Plasmid

#109560

Purpose

Multisite gateway vector for 5' tagging with early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

early endosome associated 1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pX330 PLIN2

Plasmid

#202196

Purpose

pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pECE-M2-GPHN

Plasmid

#31665

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Gephyrin (GPHN Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

Plasmid

#89874

Purpose

expresses DRD2-iTango component in mammalian cells for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

Human synapsin

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pUNIV-SIG-5HTR4D100A

Plasmid

#16312

Depositor

Bruce Conklin

Article

Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

Insert

h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Added FLAG tag; Aspartic Acid 100 changed to Alan…

Available Since

Jan. 3, 2008

Availability

Academic Institutions and Nonprofits only
pmiCas9-msa

Plasmid

#164833

Purpose

miCas9-msa fusion protein expressing plasmid

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miCas9-msa

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

contains mSA sequence range is 5106-5447 and Brex…

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

Plasmid

#163169

Purpose

Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

Non-targeting

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CD 44 pGL4basic

Plasmid

#78135

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

CD 44 (CD44 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CD44

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42

Plasmid

#109531

Purpose

Muscle specific zebrafish expression of mKate2 fused to CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42-DN

Plasmid

#109532

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneoLuc-PP2A

Plasmid

#128349

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP2A (PTPA Human)

Tags

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRELA

Plasmid

#83944

Purpose

Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgRELA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2-TP53

Plasmid

#111089

Purpose

pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Insert

TP53 (TP53 Human)

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-CDC42-DN

Plasmid

#109584

Purpose

Multisite gateway vector for 3' tagging with dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-sgCCR5

Plasmid

#83930

Purpose

Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCCR5

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge