We narrowed to 4,947 results for: mcs

1. pET21-pelB-LaG-41-4GS-LaG-2

   Plasmid

   #172766

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41-4GS-LaG-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pODN-AAVS1-mNG-CAAX

   Plasmid

   #183870

   Purpose

   Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dynamin-I-K44A-6His/Flag

   Plasmid

   #32696

   Depositor

   Robert Lefkowitz

   Article

   Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

   Insert

   Dynamin I (Dnm1 Rat)
   

   Tags

   Flag and His6

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pHLP700

   Plasmid

   #203742

   Purpose

   C-terminal tagging of target proteins with AID*/3xHA degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain codons 71-114
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xHA

   Promoter

   none

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-HypaCas9-RHOA_sgRNA

   Plasmid

   #183878

   Purpose

   pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA sgRNA spacer (RHOA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_ER-RE_MLPmin_BC1065-luc2

   Plasmid

   #227111

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_GR-RE_MLPmin_BC1094-luc2

   Plasmid

   #227114

   Purpose

   Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-LaG-18

   Plasmid

   #172763

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-18
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

   Plasmid

   #184952

   Purpose

   Donor for both PiggyBac and Cp36 Recombinase

   Depositor

   Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   PuroR, P2A, mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN-CAG-mRFP-PCNA pc2729

    Plasmid

    #166040

    Purpose

    Expresses mRFP tagged human PCNA in mammalian cells.

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-10

    Plasmid

    #172743

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-10
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaG-42

    Plasmid

    #172758

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-42
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. G1397 DddAtox-C–SaKKH-Cas9(D10A)

    Plasmid

    #157840

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    CRISPR

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV7.1_3xFLAG-TAOK3

    Plasmid

    #172993

    Purpose

    constitutive expression of FLAG-tagged TAOK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK3 (TAOK3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-NLS-AcrIIA4

    Plasmid

    #113038

    Purpose

    AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SV40 NLS-AcrIIA4
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-6

    Plasmid

    #172741

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-6
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV miRNA control plasmid

    Plasmid

    #31508

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pODN-H2BC11-mR2

    Plasmid

    #183866

    Purpose

    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mRuby2

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pODN-mR2-MYH10

    Plasmid

    #183869

    Purpose

    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG-41

    Plasmid

    #172757

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge