59,458 results

1. Flag-PML IV/pRK5

   Plasmid

   #59742

   Purpose

   Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

   Depositor

   Xiaolu Yang

   Article

   Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

   Insert

   PML isoform IV (PML Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIH1-puro-STAT3 shRNA

   Plasmid

   #26596

   Depositor

   Frank Sinicrope

   Article

   Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

   Insert

   STAT3 shRNA (STAT3 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_ST-SBP-EGFP

   Plasmid

   #65264

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-10:LMNB1-mEGFP

   Plasmid

   #87422

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HaloTag-Ubiquitin Fusion Vector

   Plasmid

   #236943

   Purpose

   Express HaloTag(R)-Ubiquitin Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   Ubiquitin (UBC Human)
   

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. SMN2E7-Fluc-Nluc-reporter

   Plasmid

   #221005

   Purpose

   Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   Fluc-SMN2E7-STOP-Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-VEE-OKS-iM

   Plasmid

   #58972

   Purpose

   RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Inserts

   Oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   cMyc (MYC Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter and IRES

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUGW-APPSwe-T2A-mCherry

   Plasmid

   #190804

   Purpose

   Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

   Depositor

   Ronald Hart

   Article

   Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

   Insert

   APP (APP Human)
   

   Use

   Lentiviral

   Mutation

   Swedish (KM670/671NL)

   Promoter

   Ubi C

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW-FIRE-pHLy

   Plasmid

   #170774

   Purpose

   Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   hUbC

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP Centrin2 (Nigg UK185)

    Plasmid

    #41147

    Depositor

    Erich Nigg

    Article

    Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

    Insert

    Centrin-2 (CETN2 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-PD-1 (Y223F, Y248F)-mGFP

    Plasmid

    #180790

    Purpose

    Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1 (Y223F, Y248F) (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    SFFV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-Rab7a-7

    Plasmid

    #54244

    Purpose

    Localization: Ras GTPase, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. LV-RFP

    Plasmid

    #26001

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-RFP (H2BC21 A. victoria, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-TREX1

    Plasmid

    #27219

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-hTimeless (pc4729)

    Plasmid

    #216861

    Purpose

    Mammalian expression vector encoding GFP tagged human Timeless

    Depositor

    Cristina Cardoso

    Article

    Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

    Insert

    Timeless (TIMELESS Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST17-UbE2C

    Plasmid

    #15779

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2C (UBE2C Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

18. deltaNp63alpha-FLAG

    Plasmid

    #26979

    Depositor

    David Sidransky

    Article

    Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

    Insert

    ΔNP63alpha (TP63 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    dominant negative isoform of p63; aa 1–587

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEFIRES-P-ACSL3-mCherry

    Plasmid

    #87158

    Purpose

    Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ACSL3 (ACSL3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF-1-alpha

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-Rab7A T22N

    Plasmid

    #28048

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    T22N

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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