We narrowed to 28,581 results for: ple

1. pLenti CMV Puro DEST (w118-1) FITM2

   Plasmid

   #107504

   Purpose

   Expresses FITM2, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   FITM2 (codon optimized)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized sequence

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. plenti-UBC- IKZF5-3xHA-pGK-PUR

   Plasmid

   #107385

   Purpose

   Lentiviral expression of IKZF5-3xHA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF5 (IKZF5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hPlekhm1-C/pIRES puro Glue (S778-A1056)

   Plasmid

   #73456

   Purpose

   Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   CBP tag, HA tag, and strep tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pLenti-VQRRA-P2A-GFP-PGK-Puro

   Plasmid

   #110864

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-CMV-cGAS R71/75E C396/7A-HA

   Plasmid

   #130920

   Purpose

   Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   human cGAS R71/75E C396/7A
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 71 and 75 mutated to glutamate; aa 396 and 397…

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

   Plasmid

   #163359

   Purpose

   Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   Ly-6G (Ly6g Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Triple ORF FRT ifgMosaic Donor (IG135)

   Plasmid

   #99626

   Purpose

   To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   PGK-Neo
   

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hPlekhm1-C/pIRES puro Glue (H987-end)

   Plasmid

   #73458

   Purpose

   Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   CBP tag, HA tag, and strep tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

10. hPlekhm1-C/pIRES puro Glue (S778-Q986)

    Plasmid

    #73457

    Purpose

    Express human C-terminal part (S778-Q986) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Stx3 triple mutant (F31A D33A E34A)

    Plasmid

    #86945

    Purpose

    Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)

    Depositor

    Thomas Weimbs

    Article

    Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. do

    Insert

    STX3 triple mutant (F31A D33A E34A) (STX3 Human)
    

    Use

    Tags

    myc-His6

    Expression

    Mammalian

    Mutation

    triple mutant (F31A D33A E34A)

    Promoter

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

    Plasmid

    #236242

    Purpose

    Lentiviral expression of SpCas9

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SpCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EP001: pLenti CMV Blast::eGFP-RC-I-1-H11

    Plasmid

    #209301

    Purpose

    Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal eGFP

    Depositor

    Adam Cohen

    Insert

    RC-I-1-H11
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EP002: pLenti CMV Blast::HaloTag-RC-I-1-H11

    Plasmid

    #209302

    Purpose

    Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal HaloTag

    Depositor

    Adam Cohen

    Insert

    RC-I-1-H11
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

    Plasmid

    #230935

    Purpose

    gRNA cloning vector encoding BFP and puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-hU6-cs1-PuroR-T2A-BFP

    Plasmid

    #230934

    Purpose

    gRNA cloning vector with direct capture sequence 1 in stem loop 2

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM

    Plasmid

    #186889

    Purpose

    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with N-terminal FLAG tag and C-terminal MAVS transmembrane domain fusion (CGAS Pongo abelii, Synthetic)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N-terminal truncation, MAVS transmembrane domain …

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-h.Rhno1 Cdk1mut7A-C-Myc-P2A-Puro

    Plasmid

    #211617

    Purpose

    Rhno1 Cdk1mut7A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S58A, S98A, S133A, T159A, T198A, S191A, and T202A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

    Plasmid

    #189633

    Purpose

    Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

    Plasmid

    #211819

    Purpose

    lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9, tracrRNA and blasticidin resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a promoter and miniU6 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge