We narrowed to 76,979 results for: Rest

1. AAVS1 intron 1 gRNA 27

   Plasmid

   #132396

   Purpose

   Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSP1-mp53AS-6xMUT

   Plasmid

   #20904

   Depositor

   Stefano Piccolo

   Article

   Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

   Insert

   p53AS (Trp53 Mouse)
   

   Expression

   Mammalian

   Mutation

   point mutation (Ser/Thr-Ala) of the 6 N-terminal …

   Available Since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

3. PC1575

   Plasmid

   #232088

   Purpose

   Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

   Depositor

   Pranam Chatterjee

   Article

   Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

   Insert

   uAb with cloning Esp3I restriction site
   

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgTnr#2/Cre

   Plasmid

   #193245

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgTnr#2 (Tnr Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-sgTnr#1/Cre

   Plasmid

   #193244

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgTnr#1 (Tnr Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pODN-H2BC11-TagBFP

   Plasmid

   #183867

   Purpose

   Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mTagBFP

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PEA1-Nuclease

   Plasmid

   #176528

   Purpose

   Delivers all prime editing nuclease components in a single plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CbH for Cas9, hU6 for gRNAs

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 intron 1 gRNA s3

   Plasmid

   #132395

   Purpose

   Targets AAVS1 intron 1, gRNA: GGGGCCACTAGGGACAGGAT, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3/ TO myc-cCE-bio (cCE-bio)

   Plasmid

   #82473

   Purpose

   Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form

   Depositor

   Daniel Schoenberg

   Article

   Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

   Insert

   Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Mouse, Synthetic)
   

   Tags

   TEV-Biotin and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid

    #50866

    Purpose

    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid

    #50875

    Purpose

    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I674C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)

    Plasmid

    #50870

    Purpose

    Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N680C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 Flag YFP hNKCC1 L737C (NT523)

    Plasmid

    #50891

    Purpose

    Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L737C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)

    Plasmid

    #50867

    Purpose

    Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Flag YFP hNKCC1 N672C (NT474)

    Plasmid

    #50873

    Purpose

    Expresses human NKCC1 N672C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N672C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 Flag YFP hNKCC1 P676C (NT443)

    Plasmid

    #50877

    Purpose

    Expresses human NKCC1 P676C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 Flag YFP hNKCC1 N731C (NT505)

    Plasmid

    #50885

    Purpose

    Expresses human NKCC1 N731C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N731C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 Flag YFP hNKCC1 V673C (NT437)

    Plasmid

    #50874

    Purpose

    Expresses human NKCC1 V673C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    V673C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 Flag YFP hNKCC1 I677C (NT467)

    Plasmid

    #50878

    Purpose

    Expresses human NKCC1 I677C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I677C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 Flag YFP hNKCC1 I678C (NT445)

    Plasmid

    #50879

    Purpose

    Expresses human NKCC1 I678C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I678C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1 Flag YFP hNKCC1 L736C (NT522)

    Plasmid

    #50890

    Purpose

    Expresses human NKCC1 L736C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L736C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 Flag YFP hNKCC1 A675C (NT441)

    Plasmid

    #50876

    Purpose

    Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 Flag YFP hNKCC1 I730C (NT504)

    Plasmid

    #50884

    Purpose

    Expresses human NKCC1 I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 Flag YFP hNKCC1 A735C (NT509)

    Plasmid

    #50889

    Purpose

    Expresses human NKCC1 A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 Flag YFP hNKCC1 S679C (NT447)

    Plasmid

    #50880

    Purpose

    Expresses human NKCC1 S679C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    S679C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 Flag YFP hNKCC1 N680C (NT468)

    Plasmid

    #50881

    Purpose

    Expresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N680C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)

    Plasmid

    #50864

    Purpose

    Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)

    Plasmid

    #50869

    Purpose

    Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    S679C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)

    Plasmid

    #50863

    Purpose

    Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I674C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)

    Plasmid

    #50868

    Purpose

    Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I677C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge