We narrowed to 8,135 results for: Dos

1. NPY-mApple

   Plasmid

   #83498

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

   Plasmid

   #173874

   Purpose

   Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-mTagBFP2-MRTF-B

   Plasmid

   #182062

   Purpose

   Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mTagBFP2-MRTF-B (Mrtfb Mouse)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-RnBarr2

   Plasmid

   #137800

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin2 (Arrb2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOCC304

   Plasmid

   #118897

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEVprotease and TwinStrep-MB…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCI-neo VPS50-13Myc

   Plasmid

   #175093

   Purpose

   13Myc tagged VPS50 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

   Insert

   VPS50 (CCDC132 Human)
   

   Use

   Tags

   13Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. untargeted Plk1 FRET sensor Myt1 substrate

   Plasmid

   #45217

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   untargeted Plk1 FRET sensor Myt1 substrate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tet-O-FUW-Nkx6.1

   Plasmid

   #45846

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

   Insert

   Nkx6.1 (Nkx6-1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-ON

   Available since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Use

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPI304

    Plasmid

    #113232

    Purpose

    extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct & pkgE-PH
    

    Use

    Dictyostelium discoideum expression vector, extra…

    Tags

    mCherry & GFP

    Expression

    Mutation

    Promoter

    act15

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL3-Basic-hCYP26A1P-E4-mCherry

    Plasmid

    #135478

    Purpose

    Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    mCherry Open reading Frame
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Short from promoter of human CYP26A1 (312 bp)

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tet-O-FUW-Zfp536

    Plasmid

    #45845

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

    Insert

    Zfp536 (Zfp536 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML107-kanR468

    Plasmid

    #162041

    Purpose

    Expresses Cas9 and contains kanR468 guide which targets the kanR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    kanR468 guide
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ki67∆repeats-mNeonGreen-IRESpuro2

    Plasmid

    #183742

    Purpose

    Expresses Ki67∆repeats-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67(lacking repeats) (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67(lacking repeats)

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Actin binding domain of Ezrin

    Plasmid

    #62381

    Purpose

    Expresses the actin binding domain of ezrin. Amino acids 552-586.

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Actin binding domain of ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains aa's 552-586

    Promoter

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK-flag-USP19 1-1290

    Plasmid

    #78579

    Purpose

    Expression flag-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-1290 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 1291 residue to stop condon

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. RnBarr1-mVenus

    Plasmid

    #137790

    Purpose

    Visualization of beta-arrestin1

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KBB364

    Plasmid

    #185083

    Purpose

    RFA1 fused to GFP under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMP-YY1_1

    Plasmid

    #36357

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pLenti6.3-efSaCas9-BlastR

    Plasmid

    #220971

    Purpose

    Expresses human codon-optimized efSaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    efSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid

    #98041

    Purpose

    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    Sa-dCas9-NLS-3xFLAG
    

    Use

    CRISPR

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    D10A + H557A

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHH0103_NR6A1_Hormone-recep

    Plasmid

    #109945

    Purpose

    Protein expression and purification of NR6A1_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR6A1_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSLIK 3xFLAG-TWIST2 neo

    Plasmid

    #197936

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-nDsRedFL-miR9/9*/124

    Plasmid

    #177807

    Purpose

    The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*/124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGImC_Empty

    Plasmid

    #92015

    Purpose

    Empty backbone, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_NR4A3_Hormone-recep

    Plasmid

    #109952

    Purpose

    Protein expression and purification of NR4A3_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR4A3_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_RORB_Hormone-recep

    Plasmid

    #109958

    Purpose

    Protein expression and purification of RORB_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RORB_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC1-FGB (FlincG2)

    Plasmid

    #49204

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG2 (PRKG1 Bovine, GFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBabe-GW-GFP

    Plasmid

    #40123

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only