We narrowed to 8,684 results for: PAN

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB9337 (pgRNA_IV-1_NatMX)

Plasmid

#161590

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFP.R400

Plasmid

#138175

Purpose

Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-GR-AKAR3

Plasmid

#173016

Purpose

Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Lyn-GR-AKAR3

Tags

EGFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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A3D-Cas9n-UGI

Plasmid

#119137

Purpose

Base editor made from APOBEC3D

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3D-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-Ura-Cen-lvl1

Plasmid

#176182

Purpose

preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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766_SMAD4 in pmiRGlo

Plasmid

#78130

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

SMAD4 (SMAD4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBS B2rescue A70D

Plasmid

#78658

Purpose

Generates B2 inserts suitable for rescue

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

Insert

B2rescue cassette

Expression

Mammalian

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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A3F-Cas9n-UGI

Plasmid

#119138

Purpose

Base editor made from APOBEC3F

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3F-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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A3C-Cas9n-UGI

Plasmid

#119136

Purpose

Base editor made from APOBEC3C

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3C-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pFP.R375

Plasmid

#138245

Purpose

Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ3: LexA-VHH-Control

Plasmid

#108241

Purpose

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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A3G-Cas9n-UGI

Plasmid

#119139

Purpose

Base editor made from APOBEC3G

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3G-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pL59-Nat

Plasmid

#140465

Purpose

CRISPR/Cas9 engineering with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ18

Plasmid

#48357

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-YFP-NLS

Plasmid

#145274

Purpose

Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MCS and YFP

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (2)

Plasmid

#185852

Purpose

Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_PTS2

Plasmid

#65676

Purpose

Expresses PTS2-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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MAGE-A12Pro pGL4basic

Plasmid

#78139

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A12 (MAGEA12 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -425/+115 of promoter

Promoter

MAGE-A12

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBM13-sd4U

Plasmid

#179691

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)

Expression

Bacterial

Mutation

Q1310R, S1330I, T1380I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFUSE-B_P2rP4

Plasmid

#97185

Purpose

For fusion gene cloning. Recombine Gene B into this Gateway Entry Vector

Depositor

Kenneth Scott

Article

Lu et al Cancer Res. 2017 May 16. pii: canres.274

Type

Empty backbone

Use

Gateway

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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P199

Plasmid

#139717

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, Luz promoter is circadian controlled

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_pAtELF4:NnLuz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

AtELF4

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ41

Plasmid

#48360

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFP.R271

Plasmid

#138220

Purpose

Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pILGFP9E3

Plasmid

#185842

Purpose

Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_H2B

Plasmid

#65702

Purpose

Expresses YNL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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