165,947 results

1. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1-Tmem192-mRFP-3xHA

   Plasmid

   #134631

   Purpose

   bait for affinity-isolation of lysosomes

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3xHA, TEV, and mRFP

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HA-mSTING

   Plasmid

   #214157

   Purpose

   Transient expression of STING

   Depositor

   Nan Yan

   Article

   Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

   Insert

   STING (Sting1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RP1B FUS 1-163

   Plasmid

   #127192

   Purpose

   Protein expression for affinity purification

   Depositor

   Nicolas Fawzi

   Article

   Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

   Insert

   FUS 1-163 (FUS Human)
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Sac-Abeta(M1-42)

   Plasmid

   #71875

   Purpose

   Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-42) (APP Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Plasmid

   #175777

   Purpose

   pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. V5-LOV-Turbo-ERM

   Plasmid

   #199664

   Purpose

   expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   SEC61B and V5

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_T2A_mCherry sgAAVS1

   Plasmid

   #192480

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-miRFP720

    Plasmid

    #197213

    Purpose

    Expresses the protein of miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    AAV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-MMLVgag-D3A-3L-dCas9-ZIM3

    Plasmid

    #240531

    Purpose

    Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing RENDER-DNMT3A-3L-dCas9-ZIM3

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-D3A-3L-dCas9-ZIM3
    

    Tags

    FLAG, HA

    Expression

    Mammalian

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. synIRES_RC25

    Plasmid

    #189970

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    synIRES_RC25
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKB225

    Plasmid

    #170015

    Purpose

    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Peptide display protein with no C-terminal peptide
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-FHC

    Plasmid

    #64874

    Purpose

    lentiviral vector with Flag, HA tags

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. OPRM1-Tango

    Plasmid

    #66464

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    OPRM1 (OPRM1 Human)
    

    Tags

    FLAG and tTA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PiggyBac PlayBack

    Plasmid

    #203312

    Purpose

    To all for PiggyBac gene insertion into Genome

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-tdTomato-CNA35

    Plasmid

    #61606

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    tdTomato and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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