We narrowed to 4,133 results for: Lta;

1. DGKD gRNA (BRDN0001147131)

   Plasmid

   #77258

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DGKD

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DGKD (DGKD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. DGKD gRNA (BRDN0001148084)

   Plasmid

   #77259

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DGKD

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DGKD (DGKD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CSNK1D gRNA (BRDN0001145070)

   Plasmid

   #76717

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CSNK1D

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CSNK1D (CSNK1D Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CSNK1D gRNA (BRDN0001146126)

   Plasmid

   #76715

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CSNK1D

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CSNK1D (CSNK1D Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. HR_G3BP1-V5-APEX2-GFP

   Plasmid

   #105284

   Purpose

   homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
   

   Use

   Human homologous recombination donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228788

   Purpose

   EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS SARS-CoV-2 D614G Spike

   Plasmid

   #185692

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 D614G Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 D614G Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutation: D614G

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR223_DLL1_WT

   Plasmid

   #82108

   Purpose

   Gateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DLL1 (DLL1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid

    #107307

    Purpose

    Encoding an SPH activation system

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG-FGF13A

    Plasmid

    #220793

    Purpose

    To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    FGF13 (FGF13 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB1677 REPAIR.t3

    Plasmid

    #176320

    Purpose

    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227898

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227899

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227890

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CROPseq-MPSV-eBFP2

    Plasmid

    #220472

    Purpose

    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

    Depositor

    Christian Krebs

    Article

    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAB1923 REPAIR.t1-S

    Plasmid

    #176321

    Purpose

    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q/E620G/Q696L, only the deaminase domain (aa …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Syn-miRFP

    Plasmid

    #108416

    Purpose

    Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Syn-Archon1-KGC-GFP-ER2

    Plasmid

    #115892

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Has Service

    AAV8

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SHIP2-ΔPS

    Plasmid

    #214901

    Purpose

    expression of the truncated variants of the SHIP2 without phosphatase domain

    Depositor

    Pavel Krejčí

    Article

    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

    Insert

    human SHIP2 delta aa 419-739 (INPPL1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    delta 419-739aa

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAB1676 REPAIR.t1

    Plasmid

    #176319

    Purpose

    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmiRFP-alpha-Tubulin-19-C

    Plasmid

    #108413

    Purpose

    Alpha-tubulin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    alpha-tubulin b (Tuba1b Mouse)
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ptdTHC_PGKneoLox2DTA.2

    Plasmid

    #112625

    Purpose

    H2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    No Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227907

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWZL Neo Myr Flag PRKCD

    Plasmid

    #20603

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PRKCD (PRKCD Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pmiRFP-Vimentin-17-C

    Plasmid

    #108414

    Purpose

    Vimentin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pD649-HAsp-DLL4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156889

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    DLL4 (DLL4 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS SARS-CoV-2 Alpha Spike

    Plasmid

    #185691

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 Alpha Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: Δ69-70, Δ145, N…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. IMPT-6213

    Plasmid

    #99333

    Purpose

    AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

    Depositor

    Raymond Stevens

    Article

    Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

    Insert

    AGTR2 (AGTR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-VHC

    Plasmid

    #112618

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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