We narrowed to 4,174 results for: Lta;

1. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MH6-uvsY

   Plasmid

   #163914

   Purpose

   Expresses uvsY for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsY (uvsY Enterobacteria phage T4)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1a-FLEx-ASAP4e-Kv-WPRE

   Plasmid

   #201033

   Purpose

   Expresses somatically enriched ASAP4e from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4b-Kv
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

   Plasmid

   #201032

   Purpose

   Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCA.66/2272

   Plasmid

   #22733

   Depositor

   Mark Magnuson

   Article

   Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

   Insert

   Lox66-pU(delta)TK-EM7Neo-Lox2272
   

   Use

   Cre/Lox; Bac recombineering

   Tags

   Expression

   Mutation

   This vector was designed for assembling gene targ…

   Promoter

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

   Plasmid

   #159086

   Purpose

   Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

   Depositor

   Jeremy Day

   Article

   Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

   Insert

   empty crRNA-acRNA backbone, dCas9-P2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hSYN, U6

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. tet-pLKO.neo_shGFP

   Plasmid

   #110470

   Purpose

   Control, silence GFP gene, doxycycline inducible, neomycin selection

   Depositor

   Sandra Martha Gomes Dias

   Article

   Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

   Plasmid

   #102596

   Purpose

   Lentivector to express STING V155M delta 368 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M delta 368

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. cacnb4 (rat) in pMT2 vector

   Plasmid

   #107426

   Purpose

   Expresses Calcium channel beta4 auxillary subunit in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

   Insert

   cacnb4 (Cacnb4 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ad MLP

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRFP-N1

    Plasmid

    #108409

    Purpose

    Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2-UAS-zmiRFP

    Plasmid

    #108415

    Purpose

    Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zmiRFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-HisD-miRFP

    Plasmid

    #108410

    Purpose

    Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmiRFP-beta-Actin-C-19

    Plasmid

    #108412

    Purpose

    Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    beta-Actin
    

    Use

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

    Plasmid

    #102594

    Purpose

    Lentivector to express STING V155M delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V155M delta 342

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP Cav2.2 HA D122A pcDNA3

    Plasmid

    #206120

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid

    #206114

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

    Plasmid

    #102595

    Purpose

    Lentivector to express STING V155M delta 354 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V155M delta 354

    Promoter

    SFFV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBKWH-Lam

    Plasmid

    #133899

    Purpose

    Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    LMNA (Lam) (LMNA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. shCCL20

    Plasmid

    #65096

    Purpose

    retroviral expression of CCL20 shRNA

    Depositor

    Pau Sancho-Bru

    Article

    Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

    Insert

    Ccl20 shRNA (Ccl20 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

22. CSNK1D gRNA (BRDN0001149348)

    Plasmid

    #76716

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSNK1D

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSNK1D (CSNK1D Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pS12<ActB.mus_2A-mCh_KI-HMEJ

    Plasmid

    #207407

    Purpose

    Donor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    Actb (Actb Mouse)
    

    Use

    CRISPR and Mouse Targeting; Hmej knockin donor

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid

    #206082

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid

    #206081

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid

    #206066

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid

    #206096

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV/B-actin

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIG-718pUC19-tNGFR-P2A-CD3D

    Plasmid

    #186100

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD3D (CD3D Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA5-LAP-MPS1-d200

    Plasmid

    #114047

    Purpose

    Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-d200 (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta 200 (deletion of NTE and TPR domains)

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge