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4930 TRV2-eTnpBc2xSV40nls-HHreRNA4PDS

Plasmid

#251823

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBc with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

2xSV40nls

Expression

Plant

Mutation

N4Y/R110K/V192L/L222I

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre-T2A-mRuby2

Plasmid

#102989

Purpose

Expresses Cre and mRuby2

Depositor

Karin Scharffetter-Kochanek

Article

Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

Inserts

Cre (site-specific recombinase)
mRuby2

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9-pAc

Plasmid

#162163

Purpose

Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

T2A-pAc

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hAdipoq-GFP

Plasmid

#176215

Purpose

Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene

Depositor

Yu-Hua Tseng

Article

Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi:

Insert

GFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pG3H-PE3max-attR1R2

Plasmid

#213049

Purpose

Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Inserts

Cas9 nickase
M-MLV Reverse Transcriptase

Use

Note: this plasmid needs helper pvs1-vir2 for rep…

Expression

Plant

Mutation

H840A, R240K, N413K,

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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4954 TRV2-eTnpBe-reRNA4PDS

Plasmid

#251818

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBe with guide targeting NbPDS with HDV at 3' end

Tags

AtFDnls

Expression

Plant

Mutation

L172G/V192L/L222I/P282V/I304R

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.LSL.EGFP

Plasmid

#100047

Purpose

AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215326

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

dNTP63 Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

dNTP63

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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4955 TRV2-eTnpBe-HHreRNA4PDS

Plasmid

#251819

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBe with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

AtFDnls

Expression

Plant

Mutation

L172G/V192L/L222I/P282V/I304R

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG03428_pLentiX1-(TRE-TagBFP-bGH)-EF1alpha-mRuby2-bGH

Plasmid

#252553

Purpose

Inducible gene and constitutive reporter with divergent syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Use

Lentiviral

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), TRE3G

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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TOP2A sgRNA1

Plasmid

#138190

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A (TOP2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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TOP2A sgRNA2

Plasmid

#138191

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A (TOP2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO

Plasmid

#73311

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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KIF1A(1-393)-GCN4-2xmCherry-EF(C)

Plasmid

#61665

Purpose

Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pGES201

Plasmid

#190197

Purpose

For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

Depositor

Yuefeng Guan

Article

Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

Insert

spCas9

Use

CRISPR

Tags

3xFlag

Expression

Plant

Mutation

plant-codon optimized

Promoter

Glycine max elongation factor 1A(pM4)

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF16B tail 458-1317

Plasmid

#134624

Purpose

Mammalian expression of human KIF16B 458-1317

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF16B (KIF16B Human)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 457 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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