We narrowed to 5,181 results for: PID;

1. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

   Plasmid

   #199356

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEG R2-L3 #BGV193

   Plasmid

   #48952

   Purpose

   For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pICH-roGFP2-ORP1

   Plasmid

   #191677

   Purpose

   Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   redox(ro)GFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. piCRg Entry

   Plasmid

   #58904

   Purpose

   Cas9/gRNA Entry expression plasmid

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   hSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pADH100

   Plasmid

   #90980

   Purpose

   NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH-chl-roGFP2-synORP1

   Plasmid

   #191701

   Purpose

   Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   chl-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH-nuc-roGFP2-synORP1

   Plasmid

   #191759

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmEGFP-C1-Nir3-LNS2

   Plasmid

   #220087

   Purpose

   C-terminal PA binding domain of Nir3

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM2 (PITPNM2 Human)
   

   Use

   Tags

   mEGFP-GGGGSHM

   Expression

   Mammalian

   Mutation

   amino acids 925-1209

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-nlsGFP-P2A

   Plasmid

   #80808

   Purpose

   nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   minimal cytomegalovirus immediate early enhancer/promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST14-SpyCatcher EQ

    Plasmid

    #35045

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher EQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pAc5 HA3-eDHFR-T2A-neo

    Plasmid

    #86396

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A neo
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A neo

    Expression

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE:iRFP670-EFS:Cre-2A-GFP

    Plasmid

    #68477

    Purpose

    Inducible lentivirus expressing iRFP670, Cre, and GFP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    iRFP670
    Cre-2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and pTRE

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. TOPO Cre-2A-GFP

    Plasmid

    #68450

    Purpose

    TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cre-2A-GFP
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL725

    Plasmid

    #140395

    Purpose

    Expresses SmtB protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-SmtB-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-GFP-Metavinculin

    Plasmid

    #107151

    Purpose

    mammalian expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

    Insert

    metavinculin (VCL Human)
    

    Use

    Tags

    (His)10-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEL1092

    Plasmid

    #195635

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEL1203

    Plasmid

    #196024

    Purpose

    Expression/purification of 6xHis-TEV-MlaC

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. px458 EQR

    Plasmid

    #101731

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid

    #133339

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-ML1N*2

    Plasmid

    #67678

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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