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lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mTurquoise2

Expression

Mammalian

Mutation

A206K

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-puro-cOVA

Plasmid

#135073

Purpose

Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

Depositor

Maria Castro

Insert

cOVA (OVAL Chicken)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAPwt

Plasmid

#210643

Purpose

Expresses the FAP (Fibroblast Activation Protein) protein.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

none

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pUCM-AAVS1-TO-hNGN2

Plasmid

#105840

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Exchangeable cassette

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pT4-CMV-GFP

Plasmid

#117046

Purpose

Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter

Depositor

Wolfgang Uckert

Insert

GFP

Use

Transposon

Promoter

CMV

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-10XUAS-IVS-Syn21-GFP-p10

Plasmid

#36432

Depositor

Gerald Rubin

Article

Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

Insert

GFP

Expression

Insect

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

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