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RPS1B-GFP (95)

Plasmid

#24039

Depositor

Michael Rout

Insert

RPS1B (RPS1B Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
RPL3-GFP (96)

Plasmid

#24040

Depositor

Michael Rout

Insert

RPL3 (RPL3 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pTK023

Plasmid

#44860

Depositor

Michael Grunstein

Article

Kitada et al Genes Dev. 2012 Nov 1;26(21):2443-55. do

Insert

ADH4-PURA3-URA3-G8-yEGFP1-CLN2PD-NLSSV40-TURA3-TG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pTCW002

Plasmid

#83522

Purpose

pheromone mediated destablised-GFP expression in yeast

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-yEGFPCLN2PEST

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAMS366-4xCDRE-GFP-PEST

Plasmid

#138658

Purpose

destabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Article

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yEGFP3-PEST

Use

Yeast reporter plasmid

Tags

Expression

Mutation

Promoter

CYC1

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

PSEC63

Available since

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

PSEC63

Available since

Availability

Academic Institutions and Nonprofits only

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deltaVP1d63C + VP2C-GFPdeg

Plasmid

#182481

Purpose

YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-GFPdeg
deltaVP1d63C

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL1 and GAL10

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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