167,150 results
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Plasmid#84561PurposepSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loopsDepositorInsertFLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
UseLentiviralTagsFlag, Suntag, oxBFP, AID (auxin-inducible degron)PromoterUbCAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-DPP4-puro
Plasmid#158451PurposeConstitutive lentiviral expression of human DPP4.DepositorAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH-Halo tag
Plasmid#154144PurposeGateway compatible mammalian expression plasmid for halo tag fusion proteinsDepositorTypeEmpty backboneTagsHalo tagExpressionMammalianAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SynA
Plasmid#174860PurposeExpresses mouse syncytin A in mammalian cellsDepositorAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HLTV-hTRIM21
Plasmid#104973PurposePurification of His-Lipoyl-hTRIM21 protein from bacteriaDepositorInsertTripartite Motif containing 21 (TRIM21 Human)
TagsHis-LipoylExpressionBacterialPromoterT7Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ki67-mNeonGreen-IRESpuro2
Plasmid#183737PurposeExpresses Ki67-mNeonGreenDepositorAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NOPlight1
Plasmid#195578PurposeExpresses NOPLight1 in mammalian cellsDepositorInsertNOPLight1
ExpressionMammalianPromoterCMVAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1553 - pAAV EF1a EGFP-KASH
Plasmid#131682PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFPDepositorInsertEGFP-KASH
UseAAVTagsKASHAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-150TAG
Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2-WPRE-bGH-polyA
Plasmid#218868PurposeMammalian expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
ExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-NRF1
Plasmid#34707DepositorAvailable SinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3
Plasmid#112005PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV5 and AAV9Insertaxon-GCaMP6s-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianPromoterhSynapsin1Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
EJ2GFP-puro
Plasmid#44025DepositorInsertEJ2GFP egfp-based chromosomal break reporter
ExpressionMammalianMutationinsertion of I-SceI, 3 frame stop, and flanking m…PromoterpCAGGSAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA3mt
Plasmid#58219PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)DepositorInsertCEPIA3mt
TagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339LPromoterCMVAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab5
Plasmid#14437PurposeMammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.DepositorAvailable SinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pST39
Plasmid#64009Purposeoriginal polycistronic cloning vector for plasmid suite (generation I)DepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJM.661
Plasmid#108532PurposeTtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.DepositorInsertTtgRAM + PTtg-YFP
ExpressionBacterialMutationTtgR: R130H A144T A148VAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT480
Plasmid#80413PurposeExpresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
ExpressionMammalianAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RLUC POLIRES FLUC
Plasmid#45642PurposeBicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.DepositorInsertRLUC - IRES - FLUC
UseLuciferaseExpressionMammalianAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mJuniper
Plasmid#219398PurposeConstitutive expression of mJuniper in E. coliDepositorInsertmJuniper
ExpressionBacterialPromoterproDpAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …Available SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+/-FLI1-C‐(K)‐DYK
Plasmid#129497PurposeMammalian expression of human FLI1DepositorAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:: pRPS5a-PE2max-NC::tHSP
Plasmid#227694PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.DepositorInsertPE2max-NC being driven by pRPS5a and terminated by tHSP
UseCRISPRExpressionPlantPromoterpRPS5aAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
Plasmid#179106PurposepCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTDepositorInsertpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
UseCRISPRExpressionMammalianAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-SpRY
Plasmid#226858PurposeExpresses ABE8e-SpRY base editor in mammalian cells with eGFP markerDepositorInsertABE8e-SpRY
ExpressionMammalianPromoterCMVAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon(-)
Plasmid#41522DepositorTypeEmpty backboneTags3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…ExpressionYeastPromoterGAL1Available SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKI_ΩBBMVP16&SRDXWUSm1
Plasmid#220349PurposeExpresses BBM-VP16 and SRDX-WUSm1 in plant cellsDepositorTagsSuperman Repression Domain X (SRDX) and VP16 tran…ExpressionPlantMutationtwo amino acid substitution in WUS-box: L255A L25…PromoterCaMV 35S and RPS5AAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-OMMjRCaMP-P2A-mito4xGCaMP6f
Plasmid#127872PurposeDual expression vector for mitochondrial matrix and outer membrane Ca2+ sensorsDepositorInsertOuter membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
ExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only