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pCMV5 Flag-Smad1

Plasmid

#14044

Depositor

Joan Massague

Publication

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HA PKB T308D S473D pcDNA3

Plasmid

#14751

Depositor

Jim Woodgett

Publication

Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

Insert

PKB DD (AKT1 Bovine)

Tags

HA

Expression

Mammalian

Mutation

T308D S473D. Constitutively active mutant.

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

23 Articles

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pCMV6M-PAK1 T423E

Plasmid

#12208

Depositor

Jonathan Chernoff

Publication

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Activated Pak1: T423E

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pSico p53

Plasmid

#12089

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating p53 shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-Casp8 C360A

Plasmid

#11818

Depositor

Guy Salvesen

Publication

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 catalytic mutant (CASP8 Human)

Tags

HA

Expression

Mammalian

Mutation

C360A

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GADD45 WT-luc

Plasmid

#8356

Depositor

Michael Greenberg

Publication

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

GADD45 promoter WT (GADD45A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p4455 FLAG-hPLIC-2

Plasmid

#8661

Depositor

Peter Howley

Publication

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Tags

FLAG

Expression

Mammalian

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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PLKO.1-Scrambled

Plasmid

#136035

Purpose

Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

Depositor

Anthony Leung

Publication

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

None (Scrambled)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Promoter

SFFV

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Mito-PyronicSF/pCMV-myc-mito

Plasmid

#124813

Purpose

Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

Depositor

Luis Felipe Barros

Publication

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CLYBL-TO-hNGN2-BSD-mApple

Plasmid

#124229

Purpose

Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

Depositor

Michael Ward

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

NGN2 (NEUROG2 Human)

Expression

Mammalian

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3 Flag-humanTRAF6 (1093)

Plasmid

#66929

Purpose

Mammalian Expression of TRAF6

Depositor

Michael Karin

Insert

TRAF6 (TRAF6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Publication

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAVS1-P-CAG-DEST

Plasmid

#80490

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Publication

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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GAL4UAS-Luciferase reporter

Plasmid

#64125

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

GAL4UAS

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GAL4UAS

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

Plasmid

#61629

Purpose

R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

Depositor

Dolf Weijers

Publication

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

mDII
ntdTomato
DII
n3xVenus

Expression

Plant

Promoter

RPS5A

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mTagBFP2-Farnesyl-5

Plasmid

#55295

Purpose

Localization: Plasma Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Farnesyl (HRAS Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLentiPGK Blast DEST JNKKTRmRuby2

Plasmid

#59154

Purpose

Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mOrange2-N1

Plasmid

#54568

Purpose

Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mOrange2

Expression

Mammalian

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mKO2-N1

Plasmid

#54625

Purpose

Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGFP Cortactin

Plasmid

#50728

Purpose

mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Insert

Cortactin (CTTN Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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CFP-Parkin

Plasmid

#47560

Purpose

CFP tagged Parkin for colocalization study

Depositor

Richard Youle

Publication

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Parkin (PRKN Human)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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5xGAL4-TATA-luciferase

Plasmid

#46756

Purpose

Luciferase reporter gene for factors with GAL4 DNA binding domains

Depositor

Richard Maurer

Publication

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Luciferase

Use

Luciferase

Promoter

minimal TATA

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pT7-EGFP-C1-HsNot1

Plasmid

#37370

Depositor

Elisa Izaurralde

Publication

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot1 (CNOT1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Publication

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pQC NLS mCherry IX

Plasmid

#37354

Depositor

Connie Cepko

Publication

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

NLS-mCherry

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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