165,744 results

1. pSLQ10878

   Plasmid

   #183964

   Purpose

   CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 TFAM-mClover

   Plasmid

   #129574

   Purpose

   Expression of mClover fused TFAM

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   TFAM (TFAM Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-Tspan4

   Plasmid

   #210323

   Purpose

   Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

   Depositor

   Li Yu

   Article

   Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

   Insert

   Tetraspanin 4 (Tspan4 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18R6KT-mini-Tn7T-Km

   Plasmid

   #64969

   Purpose

   mini-Tn7 base vector with transcriptional terminator and oriT

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pZE27GFP

   Plasmid

   #75452

   Purpose

   Plasmid for constitutive expression of gfp

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp
   

   Expression

   Bacterial

   Promoter

   placIq promoter

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

   Plasmid

   #190181

   Purpose

   Fluorescent reporter vector to visualize all of cell cycle phases.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   iRFP
   hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
   TagBFP2
   hGeminin(1/110)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX.TRE3G.AdProt

   Plasmid

   #131687

   Purpose

   A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Insert

   Adenovirus Protease
   

   Use

   Lentiviral

   Promoter

   TRE3GV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW-PK-hLC3

    Plasmid

    #61460

    Purpose

    Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

    Depositor

    Isei Tanida

    Article

    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

    Insert

    PK-hLC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-eGFP

    Plasmid

    #36083

    Purpose

    3rd generation lentiviral transfer plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-D1R

    Plasmid

    #89160

    Purpose

    Express vaccinia virus D1R in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D1R
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Expression

    Bacterial

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FRT-EF1a-TVA-2A-N2cG

    Plasmid

    #172361

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-Con/Fon TVA-mCherry

    Plasmid

    #131779

    Purpose

    INTRSECT 2.0

    Depositor

    Karl Deisseroth

    Article

    Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

    Insert

    TVA-mCherry
    

    Use

    AAV; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    nEF

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pvPE-V4-Puro

    Plasmid

    #240290

    Purpose

    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.

    Depositor

    Lusheng Huang

    Article

    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

    Insert

    pvPE-V4-Puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti PGK Puro DEST (w529-2)

    Plasmid

    #19068

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid

    #106174

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1 and AAV9

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3puro-mGFP

    Plasmid

    #225446

    Purpose

    Express mEGFP-fusion protein of interest

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. cytoplasmic NAD+ biosensor

    Plasmid

    #186787

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBITE-CD19-CD3

    Plasmid

    #190677

    Purpose

    Encodes a modularized form of HD37-OKT3 bispecific antibody

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Modularized-HD37-modularized-OKT3-P2A-NeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV mRuby3-Galectin-3

    Plasmid

    #215375

    Purpose

    Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mRuby3 tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    LGALS3 (LGALS3 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pD864-LacZ

    Plasmid

    #109380

    Purpose

    Rhamnose inducible LacZ expression. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pRHAM-LacZ
    

    Expression

    Bacterial

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-HA-Sumo1

    Plasmid

    #21154

    Purpose

    Mammalian expression of human Sumo1 with HA tag

    Depositor

    Junying Yuan

    Article

    Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

    Insert

    pcDNA3-HA-Sumo1 (SUMO1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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