We narrowed to 2,527 results for: T2A

Showing: 2181 - 2200 of 2527 results

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MOBE4

Plasmid

#219944

Purpose

Mammalian expression of MOBE4 protein (apt-CBE-SL3_PT2A_apt-ABE8.20)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-SL3_PT2A_apt-ABE8.20

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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MOBE2

Plasmid

#219942

Purpose

Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-3'end_PT2A_apt-ABE8.20

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSN993

Plasmid

#219961

Purpose

Expresses CMT2A mutant version of human KIF1Bß in C. elegans

Depositor

Shinsuke Niwa

Insert

unc-104 promoter::human KIF1Bß(Q98L) (KIF1B Nematode, Human)

Expression

Worm

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

Plasmid

#178074

Purpose

Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT2A (MZT2A Human)

Tags

PreScission-mEGFP and ZZ-TEV

Expression

Insect

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTet-O-Ngn2-puro

Plasmid

#52047

Purpose

3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Article

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Ngn2 (Neurog2 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mCherry-EGFP-LC3B

Plasmid

#170446

Purpose

Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

mCherry-GFP-LC3 (MAP1LC3B Human)

Use

Lentiviral

Promoter

EF1a

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-mCherry

Plasmid

#104833

Purpose

3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

eFFly
mCherry

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_P2A_Puro

Plasmid

#211364

Purpose

Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

Depositor

Jacques Neefjes

Article

Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2_2A_Sox11-PURO

Plasmid

#226284

Purpose

Tetracycline-inducible expression of Ngn2 and Sox11 for direct reprogramming of canine fibroblasts to induced-motor neurons

Depositor

Susannah Sample

Article

Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

Insert

NGN2:T2A:SOX11 (SOX11, NEUROG2 Human)

Use

Lentiviral

Tags

NGN2 and SOX11 linked via T2A

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225703

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

Plasmid

#85799

Purpose

donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

Plasmid

#132389

Purpose

Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

human TauWT 2N4R-EGFP (MAPT Synthetic, Human)

Use

CRISPR and Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

TREtight

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

Plasmid

#225699

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-SOK

Plasmid

#20327

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225702

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-extGluc-mKate-NGFR

Plasmid

#104831

Purpose

extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

mKate
NGFR

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PEA1-Puro-HEK3 CTT ins

Plasmid

#171996

Purpose

Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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