We narrowed to 2,367 results for: clu

1. GG-EBNA-MIR302-7guides-PGK-Puro

   Plasmid

   #201960

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

   Depositor

   Timo Otonkoski

   Article

   Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

   Insert

   MIR302-7g-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

   Plasmid

   #146499

   Purpose

   Insect Expression of DmGW182

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmGW182 (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-pSMANTIS_WT_1kb

   Plasmid

   #194187

   Purpose

   Includes the promoter (1kb) of SMTS (SMANTIS, MANTIS, AK125871)

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SMANTIS promoter (1kb)

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

   Plasmid

   #190154

   Purpose

   Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp3 (Vamp2 Rat)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only rat Vamp3 AA #1-81

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS97splitRBP_S2-Enz

   Plasmid

   #183768

   Purpose

   Expresses SHIP2-Enzyme domain flanked by RBP

   Depositor

   William Kerr

   Article

   Sudan et al Front Immunol. 2022 May 4;13:830961. doi

   Insert

   SHIP2 (INPPL1 Human)
   

   Use

   Tags

   6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

   Expression

   Bacterial

   Mutation

   includes aa K414 to aa V872 only

   Promoter

   T7lac

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pODN-H2BC11-TagBFP

   Plasmid

   #183867

   Purpose

   Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mTagBFP

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmAGO2-RC_B

   Plasmid

   #145981

   Purpose

   Insect Expression of DmAGO2-RC

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

   Insert

   DmAGO2-RC (AGO2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   A deletion of AA 51-56 and an insertion of 23 AA …

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. vPyACR_2164382

   Plasmid

   #153029

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_2164382
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_2164382 was C-terminally truncated to incl…

   Promoter

   CMV (+ enhancer)

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. vPyACR_21821

   Plasmid

   #153030

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_21821
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_21821 was C-terminally truncated to includ…

   Promoter

   CMV (+ enhancer)

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Puro-NCOA7g3 (BB24)

    Plasmid

    #139459

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAN19-TIR1-GUS-NOSt

    Plasmid

    #108547

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAN19-ccvTIR1-GUS-NOSt

    Plasmid

    #108548

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Changed F79 to G

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-BiFC-Venus

    Plasmid

    #105804

    Purpose

    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    VN173 (I152L); VC155

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CCR2-DuET

    Plasmid

    #213198

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR2 (CCR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid

    #71390

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MLRV

    Plasmid

    #118069

    Purpose

    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Inserts

    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pgk

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. DNA-PKcs PQR>ala

    Plasmid

    #83319

    Purpose

    human DNA-PKcs PQR>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 phosphorylation sites in the PQR cluster substi…

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHSN501

    Plasmid

    #50589

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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