165,947 results

1. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Promoter

   EF1 alpha

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

   Plasmid

   #225697

   Purpose

   Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (WT) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mCherry

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pepper-fused sgRNA

   Plasmid

   #217520

   Purpose

   Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   Pepper-fused sgRNA for spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNatB (pACYCduet-naa20-naa25)

   Plasmid

   #53613

   Purpose

   Allows expression of the fission yeast NatB complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

   Inserts

   naa20 (naa20 Fission Yeast)
   naa25 (naa25 Fission Yeast)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p28-2iDMD-puro

   Plasmid

   #88895

   Purpose

   28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dystrophin (DMD Human)
   luciferase
   puromycin resistance enzyme
   mCherry
   

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. GZnP2 in pcDNA3.1+

   Plasmid

   #161739

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

   Insert

   GZnP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIp10 - γmGFP (γmGFP based)

   Plasmid

   #163119

   Purpose

   Expresses in Candida albicans

   Depositor

   Patrick Van Dijck

   Article

   Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

   Insert

   gamma mGFP
   

   Use

   Candida albicans expression

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

   Plasmid

   #69901

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cyto-RA

    Plasmid

    #209860

    Purpose

    Dimerization dependent fluorescent protein RA fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    NES sequence NLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCV-Cas9

    Plasmid

    #123643

    Purpose

    Expresses Cas9 with an N-terminal HUH-tag called PCV2

    Depositor

    Wendy Gordon

    Article

    Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

    Inserts

    PCV2 (ORF1 )
    Cas9
    

    Tags

    (EAAAK)8 linker and His6 + SUMO

    Expression

    Bacterial

    Mutation

    2-116

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. c-Jun

    Plasmid

    #187902

    Purpose

    Expresses c-Jun in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    c-Jun (JUN Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-scFv-GCN4-mStayGold-GB1-dWPRE

    Plasmid

    #231598

    Purpose

    Express scFv_GCN4-mStayGold for SunTag translation imaging

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    scFv-GCN4-mStayGold
    

    Use

    Lentiviral

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-hSyn-RFP

    Plasmid

    #22909

    Depositor

    Edward Callaway

    Article

    Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

    Has Service

    Cloning Grade DNA

    Insert

    human synapsin I promoter driving DsRedExpress (SYN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX_DSB_Spectrum-V2

    Plasmid

    #192475

    Purpose

    Lentiviral DSB Spectrum V2 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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