161,439 results

1. pBluescript II SK--POLQ

   Plasmid

   #64873

   Purpose

   cloning vector for human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-CYLD-WT

   Plasmid

   #15506

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   CYLD (CYLD Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINE

   Plasmid

   #26043

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His-lacZ-KHHHHHH
   

   Use

   Tags

   KHHHHHH and MAHHHHHHSSG

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. ChR2-2A-Halo

   Plasmid

   #22047

   Purpose

   Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

   Depositor

   Edward Boyden

   Article

   Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

   Insert

   ChR2-GFP-2A-Halo-YFP
   

   Use

   Tags

   GFP and YFP

   Expression

   Mammalian

   Mutation

   Note that there is a S147R mut in YFP. This mutat…

   Promoter

   CMV

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK7WGF2::hCas9

   Plasmid

   #46965

   Purpose

   Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   hCas9
   

   Use

   CRISPR; Plant expression

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   35S

   Available since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-hARFGAP2-HA

   Plasmid

   #206410

   Purpose

   Mammalian expression of hARFGAP2

   Depositor

   Hye-Won Shin, Kazuhisa Nakayama

   Article

   Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

   Insert

   ARFGAP2 (ARFGAP2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJQ200SK

   Plasmid

   #78497

   Purpose

   Suicide vector for gene replacement

   Depositor

   Michael Hynes

   Article

   Quandt et al Gene. 1993 May 15;127(1):15-21.

   Type

   Empty backbone

   Use

   Suicide plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xMma PylT sfGPF 150TAG

   Plasmid

   #154766

   Purpose

   plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, U25C in PylT

   Promoter

   EF1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-SorLA-v2

   Plasmid

   #154900

   Purpose

   Expresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   SORL1 (SORL1 Human)
   

   Use

   Tags

   Venus fragment 2 (v2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRPML1-HA

    Plasmid

    #18825

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6HisVDAC1/pCDNA3

    Plasmid

    #197320

    Purpose

    Plasmid expressing an antigen targeting the VDAC1 transporter

    Depositor

    Dan Minor

    Insert

    VDAC1 (VDAC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDA019

    Plasmid

    #131452

    Purpose

    control expression vector

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFRT/TO/HIS/FLAG/HA-PRDX1

    Plasmid

    #38086

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PRDX1 peroxiredoxin 1 (PRDX1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-HA-hTAF1

    Plasmid

    #17997

    Depositor

    Robert Tjian

    Article

    Ruppert et al Nature. 1993 Mar 11. 362(6416):175-9.

    Insert

    hTAF1 (TAF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS420

    Plasmid

    #11259

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKS diaCas9_sgRNA

    Plasmid

    #74923

    Purpose

    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

    Depositor

    Per Winge

    Article

    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Cas9 module and sgRNA module

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRIS-PITChv2-FBL

    Plasmid

    #63672

    Purpose

    PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    EGFP-2A-PuroR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Promoterless

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-BI

    Plasmid

    #126475

    Purpose

    High-copy number vector for the co-expression of two genes in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.CMV.LacZ.bGH

    Plasmid

    #105531

    Purpose

    AAV expression of LacZ from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV5 and AAV8

    Insert

    LacZ
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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