We narrowed to 6,121 results for: cas9 expression plasmid

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sgRNA1_MYOD1

Plasmid

#64136

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA2_MYOD1

Plasmid

#64137

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_MYOD1

Plasmid

#64139

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA3_MYOD1

Plasmid

#64138

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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U6-spTRE3G-CMV-mTagBFP2

Plasmid

#102854

Purpose

A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sp-TRE3G-sgRNA; mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAGFP

Plasmid

#58255

Purpose

U6 promoter-driven single guide RNA complementary to eGFP

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAGFP cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAS1

Plasmid

#58252

Purpose

U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAS1 cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#206994

Purpose

Plasmid expressing both SpCas9 and SaCas9

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Insert

SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )

Tags

P2A

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-SlugABE-NNG

Plasmid

#214356

Purpose

Expresses SlugABE-NNG

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG

Use

AAV

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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gh123

Plasmid

#106790

Purpose

expression of gRNA targeting ST8SIA3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA3 (ST8SIA3 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTrex-n-eGFP

Plasmid

#62544

Purpose

Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

Depositor

Rick Tarleton

Article

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Insert

eGFP

Use

(1)trypanosoma cruzi expression of egfp (2) genom…

Tags

None

Mutation

None

Promoter

Ribo-HX1

Available Since

April 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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gh19

Plasmid

#106703

Purpose

expression of gRNA targeting GALNT19

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT19

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh171

Plasmid

#106832

Purpose

expression of gRNA targeting UGCG

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGCG (UGCG Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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epiCRISPRn

Plasmid

#135961

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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gh54

Plasmid

#106727

Purpose

expression of gRNA targeting ST3GAL6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST3GAL6 (ST3GAL6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEL675

Plasmid

#196822

Purpose

dLrCas9 gene expression repression plasmid in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL677

Plasmid

#196824

Purpose

LrCas9 gene expression activation plasmid in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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epiCRISPRe

Plasmid

#135962

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only
pJM149

Plasmid

#134168

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC E118A R129A, E. coli codon optimized, CRISPR…

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJM147

Plasmid

#134167

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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gh174

Plasmid

#106835

Purpose

expression of gRNA targeting UGT8

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGT8 (UGT8 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO-mCherry_Dicer gRNA

Plasmid

#215279

Purpose

Expresses Dicer gRNA in a Cre dependent mcherry vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Dicer control gRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

Plasmid

#215282

Purpose

Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Grin1 control gRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL676

Plasmid

#196823

Purpose

dLrCas9 gene expression activation plasmid in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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