We narrowed to 18,832 results for: Tec

1. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #50946

   Purpose

   An empty gRNA expression vector

   Depositor

   Kosuke Yusa

   Article

   Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBintE

   Plasmid

   #48136

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b-RvLEAMshort

   Plasmid

   #218122

   Purpose

   Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

   Depositor

   Ci Ji Lim

   Article

   Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

   Insert

   RvLEAMshort
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Truncated form (58-181aa)

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MS2-epegRNA-Dnmt1

   Plasmid

   #211372

   Purpose

   Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MS2-epegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1119 (pDonor)

   Plasmid

   #160732

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVH1002-Tier2(pUC ori)

   Plasmid

   #169600

   Purpose

   Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY1314_RADARS_Luciferase

   Plasmid

   #193191

   Purpose

   Express Gaussia Luciferase conditional upon RNA transcript

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   RADARS Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ePPE-spG

   Plasmid

   #183096

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nspGCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpGCas9

   Promoter

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB6364

    Plasmid

    #106151

    Purpose

    EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFYF1320 EGFP Site#1

    Plasmid

    #47511

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-U6-epegRNA eBAR1-SV40-puro

    Plasmid

    #240535

    Purpose

    Lentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

    Depositor

    Wensheng Wei

    Article

    Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

    Insert

    epegRNA-eBAR1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

    Plasmid

    #191161

    Purpose

    Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP.t2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3-Neo-EF1a-GFP

    Plasmid

    #69134

    Purpose

    Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Type

    Empty backbone

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U3MSCV

    Available Since

    Jan. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTK313

    Plasmid

    #59570

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C1(1-29)-TurboID-V5_pLX304

    Plasmid

    #107175

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJRH-1328 LV EF1a-CD20 IRES-EGFP

    Plasmid

    #201918

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD20
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mRNA-CleanCap entry vector

    Plasmid

    #188249

    Purpose

    Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTK317

    Plasmid

    #59571

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PrimeRoot.v2C-Cre

    Plasmid

    #201739

    Purpose

    For DNA insertion test in protoplast

    Depositor

    Caixia Gao

    Article

    Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    ePPE,Cre
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBT277

    Plasmid

    #122608

    Purpose

    express evoCDA1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoCDA1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F23S A123V I195F

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLX_311-Cas9

    Plasmid

    #96924

    Purpose

    for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

    Depositor

    John Doench, David Root, William Hahn

    Article

    Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    3xFlag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. nls-EGFP

    Plasmid

    #67652

    Purpose

    Mammalian expression vector driving nls-EGFP.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    nls-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Expression

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-6xHis-(pos36)GFP

    Plasmid

    #62937

    Purpose

    Expression of 6xHis-(+36)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (pos36)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT7-TaGu-WT-3xF-ORF

    Plasmid

    #238487

    Purpose

    For production of C-term flag-tagged TaGu R2 ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SaABE8e

    Plasmid

    #138500

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEF-HA/Net1A

    Plasmid

    #28262

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1A (Net1 Mouse)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pCAG-scFvGCN4sfGFPTET1CD

    Plasmid

    #82561

    Purpose

    Expression vector for antibody-sfGFP-TET1CD.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLX-TRV2

    Plasmid

    #180516

    Purpose

    Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker

    Depositor

    Jose-Antonio Daros

    Article

    Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

    Insert

    Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S CaMV

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-Flpo

    Plasmid

    #125576

    Purpose

    Expresses Flpo under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    FLPo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge