We narrowed to 18,363 results for: Tec

1. pCaRNA3

   Plasmid

   #86088

   Purpose

   Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA3
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-ms-Sox7

   Plasmid

   #50780

   Purpose

   retroviral expression of mouse Sox7

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Sox7 (Sox7 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBK-YE1-BE3

   Plasmid

   #85174

   Purpose

   Expresses YE1-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   YE1-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PDGFR-TMD (MTK 3B) (pZYW025)

   Plasmid

   #194218

   Purpose

   Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PDGFR TMD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T2A-mCherry (MTK 4a) (pAN3844)

   Plasmid

   #194223

   Purpose

   Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   T2A-mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

   Plasmid

   #194229

   Purpose

   Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK-LCB1-SynNotch-T2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

   Plasmid

   #193968

   Purpose

   Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   hPol eta (POLH Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-c/o NL4-3 Env delta CT

   Plasmid

   #139285

   Purpose

   HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   codon optimized HIV-1 Env delta cytoplasmic tail
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of cytoplasmic tail after residue 710

   Promoter

   CMV IE

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SaCas9 TadDE

   Plasmid

   #193846

   Purpose

   Express TadDE (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE with SaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBK-YEE-BE3

    Plasmid

    #85177

    Purpose

    Expresses YEE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT2-CAG-fGFP

    Plasmid

    #108714

    Purpose

    Transposon donor plasmid for long-term expression of membrane targetted GFP

    Depositor

    Madeline Lancaster

    Article

    Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

    Insert

    EGFP
    

    Use

    Tags

    6xHis and farnesylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-(-30)GFP-9xGGS-Cre-6xHis

    Plasmid

    #62372

    Purpose

    Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP-9xGGS-Cre-6xHis
    

    Use

    Tags

    (-30)GFP and 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CP1041-ABE8e

    Plasmid

    #138493

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-Flp-DOG-NW

    Plasmid

    #75469

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Flp-DOG
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ND4.2-Left TALE-G1397-N-DddA6

    Plasmid

    #179680

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q1310R, S1330I, T1380I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGWB412

    Plasmid

    #74806

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-GfaABC1D-GRAB_eCB2.0

    Plasmid

    #164609

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    GfaABC1D

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA011

    Plasmid

    #215973

    Purpose

    Fragmid fragment: (N' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA010

    Plasmid

    #216038

    Purpose

    Fragmid fragment: (C' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    UGI_v1.1; NpNLS_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA013

    Plasmid

    #215975

    Purpose

    Fragmid fragment: (N' terminus) for A>G base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFEndoNA

    Plasmid

    #174724

    Purpose

    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB543

    Plasmid

    #74877

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTS1051-Tier1-PhCMV-4xCox8

    Plasmid

    #169490

    Purpose

    Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGWB408

    Plasmid

    #74802

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSL2669 (pTnsABf_Vch)

    Plasmid

    #200897

    Purpose

    Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAGK111

    Plasmid

    #228126

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Use

    Tags

    Antares

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LbABE8e(TadA-8e V106W)

    Plasmid

    #138508

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-dLbCas12a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTRE3G-iRFP-B1-timestamp_A

    Plasmid

    #159924

    Purpose

    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pY027

    Plasmid

    #84742

    Purpose

    Expresses huLbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pU6-Sp-gRNA-ALB_DF_B358c_attB

    Plasmid

    #182146

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    ALB_B358c_attB pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pU6-Sp-gRNA-ALB_DF_A277c_attB

    Plasmid

    #182145

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    Alb_A277c_attB pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-RAB-EKARev(T/A)

    Plasmid

    #118475

    Purpose

    Negative-control mutant for RAB-EKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

    Plasmid

    #191145

    Purpose

    Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3UTR(Bdnf)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. eNme2-C TadDE

    Plasmid

    #193842

    Purpose

    Express TadDE (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadDE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCellFree_G03 GDI2

    Plasmid

    #67093

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GDI2 (GDI2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

41. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

    Plasmid

    #191141

    Purpose

    Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-W:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    UAG>UGG (stop>Trp)

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid

    #217333

    Purpose

    piggyBac expression of denAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    denAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    D908A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pShoHELIX-sgRNA_entry (BO3)

    Plasmid

    #181784

    Purpose

    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSin-GFP-Fg

    Plasmid

    #174307

    Purpose

    Lentiviral expression of GFP

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GFP (eGFP Aequorea victoria)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. shCDK4/6(1)

    Plasmid

    #73554

    Purpose

    vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

46. pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115209

    Purpose

    Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PbuCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBK-EQR-BE3

    Plasmid

    #85172

    Purpose

    Expresses EQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAsCpf1(TYCV)(BB) (pY211)

    Plasmid

    #89352

    Purpose

    Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K607R

    Promoter

    CBh

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLV-SI-121

    Plasmid

    #131126

    Purpose

    A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pRN110

    Plasmid

    #127228

    Purpose

    CmYLCV::STM::35S terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    STM (STM A. tumefaciens, Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge