165,947 results

1. miniCMV-tdTomato-tDeg

   Plasmid

   #217521

   Purpose

   Expresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   tdTomato-tDeg fluorogenic protein
   

   Expression

   Mammalian

   Promoter

   miniCMV

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TEV Protease-mCherry

   Plasmid

   #243793

   Purpose

   Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   TEV protease
   

   Expression

   Mammalian

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. IRF3-V5

   Plasmid

   #169775

   Purpose

   Express IRF3 in mammalian cells

   Depositor

   Sung-Liang Yu

   Article

   Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

   Insert

   Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)
   

   Tags

   V5 tag

   Expression

   Bacterial and Mammalian

   Available Since

   July 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 hygro

   Plasmid

   #24150

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

   Depositor

   Bob Weinberg

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0589

   Plasmid

   #142653

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXO1 (FOXO1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Sac-Abeta(M1-40)

   Plasmid

   #71876

   Purpose

   Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-40) (APP Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

   Plasmid

   #192617

   Purpose

   AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   FLEX-RiboL1-jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pShuttle-CMV

   Plasmid

   #16403

   Purpose

   Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB179-HotPETase

   Plasmid

   #218701

   Purpose

   Encodes codon optimized HotPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   HotPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid

    #46912

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-VP64-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    3xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Promoter

    LTR and PGK

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. His6-TEV-CRBN-midi

    Plasmid

    #215330

    Purpose

    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

    Depositor

    Alessio Ciulli

    Article

    Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

    Insert

    CRBN (CRBN Human)
    

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 41-187 followed by a Gly-Ser…

    Promoter

    lacI

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available Since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. hNTo1-qgRNA-pYJA5

    Plasmid

    #217781

    Purpose

    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. hNTo2-qgRNA-pYJA5

    Plasmid

    #217782

    Purpose

    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_429

    Plasmid

    #179098

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0500

    Plasmid

    #143709

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPARGC1A (PPARGC1A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

    Plasmid

    #163476

    Purpose

    Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    iCre
    

    Use

    AAV

    Promoter

    Beta Globin minimal promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Halo-KDEL

    Plasmid

    #124316

    Purpose

    Expresses ER specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Tags

    KDEL Seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-Tol2

    Plasmid

    #31831

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Use

    Cloning vector

    Promoter

    Lac

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAM5188

    Plasmid

    #120087

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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