161,641 results

1. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDx_mScarlet3

   Plasmid

   #189754

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LCV2 cGAS KO

   Plasmid

   #217444

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human cGAS

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   sgRNA targeting cGAS (CGAS Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MP6

   Plasmid

   #69669

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ER-HA-mNG21-10

   Plasmid

   #141165

   Purpose

   Split Fluorescent Reporter for ER Retrograde Trafficking

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   ER-HA-mNG21-10-KDEL
   

   Use

   Lentiviral

   Tags

   Signal Sequence, KDEL and HA

   Expression

   Mammalian

   Mutation

   K128M, S142T, R150M, G172V and K213M

   Promoter

   Ef1a

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209665

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-NTOM20-mVenus-Zdk1

   Plasmid

   #81010

   Purpose

   Expression of Zdk1 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Use

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-NUP50-N-10

   Plasmid

   #55111

   Purpose

   Localization: Nuclear Pore, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   NUP50 (NUP50 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-GP

   Plasmid

   #60511

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDARMO_CMVT_3xFLAG_BIRC6

    Plasmid

    #197967

    Purpose

    Mammalian cell expression of wt 3xFLAG-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L1332V

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK33/Cas9

    Plasmid

    #133781

    Purpose

    Expresses drosophila-optimized Cas9

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Insert

    Drosophila-optimized Cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX_305-N-dTAG

    Plasmid

    #91797

    Purpose

    Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-EKAR4

    Plasmid

    #174437

    Purpose

    Improved cyan/yellow FRET-based ERK kinase activity reporter.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    EKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3007

    Plasmid

    #144483

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMAD1 (SMAD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-flex-ReaChR-citrine

    Plasmid

    #50955

    Purpose

    flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF2998

    Plasmid

    #144474

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-HA-HA-human CMYC S62A

    Plasmid

    #203436

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    S62A

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. DH-BB(5'SIN;TE12ORF)

    Plasmid

    #72309

    Purpose

    Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus

    Depositor

    Anthony Zador

    Insert

    DH-BB(5'SIN;TE12ORF)
    

    Use

    Sindbis virus helper construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEmerald-GPAT4152-208

    Plasmid

    #134468

    Purpose

    GPAT4152-208 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    GPAT4 (GPAT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GPAT4, amino acids 152-208

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiPGK Puro DEST PKAKTRClover

    Plasmid

    #59153

    Purpose

    Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA (PRKACA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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