8,734 results

1. pCMV-sadCas9-VP64

   Plasmid

   #115790

   Purpose

   An AAV vector expressing S. aureus dCas9 fused to VP64.

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   Sa-dCas9
   

   Use

   AAV

   Tags

   NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIP-BSR-GFP11

   Plasmid

   #68716

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   bsr
   

   Use

   Retroviral

   Tags

   FLAG and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-M2

   Plasmid

   #44170

   Depositor

   John Olsen

   Article

   Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

   Insert

   Influenza A virus M2 matrix gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Use

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRLSIN.cPPT.PGK-GFP.WPRE

   Plasmid

   #12252

   Purpose

   3rd generation lentiviral backbone

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT/TO/HIS/FLAG/HA-ALKBH5

   Plasmid

   #38073

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Calreticulin-N-16

   Plasmid

   #55006

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Calreticulin (CALR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneoEGFP

   Plasmid

   #46949

   Purpose

   GFP in Promega pCIneo (uses CMV promoter). Superior to pfwB for some purposes

   Depositor

   John Schiller

   Article

   Buck et al Curr Protoc Cell Biol. 2007 Dec;Chapter

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pet28a_Synthetic_Human_H2A.1

    Plasmid

    #42634

    Depositor

    Joe Landry

    Insert

    Human H2A.1 (H2AC13 Synthetic, Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Codon Optimized

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHLmMBP-10

    Plasmid

    #72348

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pER-mScarlet3_N1

    Plasmid

    #189772

    Purpose

    Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    ER-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX552

    Plasmid

    #60958

    Purpose

    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Inserts

    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin and U6

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEX-GFP

    Plasmid

    #28304

    Depositor

    Edward Boyden

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBB540

    Plasmid

    #27393

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE, clpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSRK2706

    Plasmid

    #78571

    Purpose

    Expresses untagged human rhinovirus 3C protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    human rhinovirus 3C protease (HRVBgp1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge