8,812 results

1. pMV306hsp

   Plasmid

   #26155

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   hsp60 promoter
   

   Use

   Mycobacteria integrating vector

   Available Since

   Nov. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. YopH (residues 164-468)

   Plasmid

   #79749

   Purpose

   This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   YopH (residues 164-468)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-hERG1a

   Plasmid

   #53052

   Purpose

   Expresses alpha subunit of hERG1a K channel in mammalian cells

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. shYAP1 # 1

   Plasmid

   #42540

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-rab7 WT

   Plasmid

   #12605

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. Proinsulin-NanoLuc in pLX304

   Plasmid

   #62057

   Purpose

   Luminescent reporter of insulin secretion

   Depositor

   David Altshuler

   Article

   Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

   Insert

   Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   Inserted NanoLuc into C-peptide of mouse Ins2 cod…

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZipGFP-Casp3

   Plasmid

   #81241

   Purpose

   A ZipGFP-based Caspase-3 protease reporter T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

   Insert

   ZipGFP with caspase cleavage sequence T2A mCherry
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL SFFV d20GFP.T2A.mTagBFP Donor

    Plasmid

    #31485

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d20BFP T2A BFP
    

    Use

    Lentiviral

    Mutation

    GFP has 20 amino acids deleted from the C terminu…

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-TetO-hNGN2-Puro

    Plasmid

    #79049

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Promoter

    TetO

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-U6-crRNA-empty

    Plasmid

    #134921

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    crRNA for CRISPR-Cas3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. SnailHA_pcDNA3

    Plasmid

    #31697

    Depositor

    Paul Wade

    Article

    Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

    Insert

    Snail (SNAI1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-luciferase

    Plasmid

    #55764

    Purpose

    luciferase reporter for in vivo bioluminescence imaging

    Depositor

    Snorri Thorgeirsson

    Article

    Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

    Insert

    CMV enhancer/chicken beta actin promoter
    

    Expression

    Mammalian

    Promoter

    CMV enhancer/chicken beta actin promoter

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18T-mini-Tn7T-Gm

    Plasmid

    #63121

    Purpose

    mini-Tn7 vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3/UBC9

    Plasmid

    #20082

    Depositor

    Edward Yeh

    Article

    Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

    Insert

    UBC9 (UBE2I Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

    Plasmid

    #153005

    Purpose

    Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (C) (G79)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-mitoTPNOX

    Plasmid

    #87854

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    mitoTPNOX
    

    Tags

    C-terminal FLAG tag with a linker and mitochondri…

    Expression

    Bacterial

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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