We narrowed to 14,168 results for: eng

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rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

Plasmid

#32479

Purpose

Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Publication

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F-GlyR-IRES-GFP

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEX-GST-AGO1

Plasmid

#24316

Depositor

Carl Novina

Publication

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO1 (AGO1 Human)

Tags

GST

Expression

Bacterial

Available since

April 7, 2010

Availability

Academic Institutions and Nonprofits only
pL2Cas9

Plasmid

#98841

Purpose

Genome engineering of virulent phages

Depositor

Sylvain Moineau

Publication

Lemay et al ACS Synth Biol. 2017 Mar 30. doi: 10.102

Insert

tracr/Cas9

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

Plasmid

#135456

Purpose

Bacterial expression of uTEV2Δ protease

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability, T30A/S153N mut…

Promoter

tac promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMOD_B2303

Plasmid

#91068

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers

Use

CRISPR

Promoter

CmYLCV

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pVSVdG-4BFP2

Plasmid

#64101

Purpose

Expresses mTagBFP2

Depositor

Ian Wickersham

Insert

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Synapsin-pm-iATPSnFR1.0

Plasmid

#102555

Purpose

expresses membrane displayed ATP sensor in neurons

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_220

Plasmid

#91113

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTRANS_220d

Plasmid

#91114

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGW004_pAAV-hU6-SapI-hTBG-Fluc-P2A-Cre

Plasmid

#256631

Purpose

AAV vector for delivery of Cas9 guide and expressing Fluc and Cre using hTBG promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

AAV

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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ePPE

Plasmid

#183095

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas9-ScaligD

Plasmid

#125688

Purpose

Gene knockout/in for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spCas9, sgRNA casette, and ScaligD

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJH124

Plasmid

#102951

Purpose

Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

Depositor

Peter Espenshade

Publication

Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

Insert

APEX2-NES

Tags

FLAG

Expression

Yeast

Promoter

GAL1

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAW014-2

Plasmid

#85612

Purpose

Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

Depositor

Perry Chou

Publication

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

ugtP-gRNA.P395T

Expression

Bacterial

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-HypaCas9

Plasmid

#108302

Purpose

pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

Plasmid

#114070

Purpose

T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

bacteria codon optimized LbCas12a with C-terminal NLS and His-tag

Tags

NLS(nucleoplasmin) and 6xHis

Expression

Bacterial

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO5d.EFS.SpCas9.P2A.BSD

Plasmid

#57821

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-BSD

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA3.1(+)-NEMOs

Plasmid

#189931

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Publication

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOs

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC78

Plasmid

#62339

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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