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pcDNA3-AKAR4-NES

Plasmid

#64727

Purpose

Cytosol-targeted, FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

Insert

AKAR4-NES

Tags

Cerulean, NES, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP8

Plasmid

#22608

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP8 (USP8 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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drebrin-YFP

Plasmid

#40359

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Chick expression

Tags

eYFP

Promoter

chick B-actin promoter with CMV enhancer

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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AV09_pDonor.EP.S1.TS

Plasmid

#100297

Purpose

AAVS1-targeting expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

PuroR.T2A.EGFP expression unit

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 236HSC WT (BRCA2)

Plasmid

#16246

Depositor

Mien-Chie Hung

Article

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

Plasmid

#176851

Purpose

lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1-5SA (YAP1 Human)

Use

Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.tRFP

Plasmid

#57819

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

Plasmid

#210020

Purpose

tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO-mini-CMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mouse b8 full length

Plasmid

#217817

Purpose

Full length mouse integrin b8 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGB8 (Itgb8 Mouse)

Tags

P2A-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pIntegrase_9

Plasmid

#60580

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_9

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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