4,176 results

1. mKO2-N1

   Plasmid

   #54625

   Purpose

   Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

   Depositor

   Atsushi Miyawaki, Michael Davidson

   Article

   Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

   Type

   Empty backbone

   Tags

   mKO2

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-D50 lamin A

   Plasmid

   #17653

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   D50 lamin A (LMNA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50 (50 aa internal deletion in globular tai…

   Promoter

   CMV

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-MCS-ASC-GFP

   Plasmid

   #73957

   Purpose

   Express human ASC with a C-terminal EGFP tag in mammalian cells

   Depositor

   Christian Stehlik

   Article

   de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

   Insert

   PYD and CARD domain containing, PYCARD (PYCARD Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-CMV-GCaMP6s-CAAX

   Plasmid

   #52228

   Purpose

   Plasma membrane targeted GCaMP6s, calcium sensor

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   GCaMP6s-CAAX
   

   Tags

   6xHis and CAAX

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIDA1

   Plasmid

   #79180

   Purpose

   A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

   Depositor

   Gen Larsson

   Article

   Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

   Insert

   Adhesin Involved in Diffuse Adherence
   

   Tags

   His and Myc

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 7TFC

   Plasmid

   #24307

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-mCherry
   

   Use

   Lentiviral

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-sgRNA expression vector

   Plasmid

   #51132

   Purpose

   For in vitro transcription of sgRNA from the T7 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad-sfGFP

   Plasmid

   #85482

   Purpose

   Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   superfolder GFP
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   Arabinose

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid

    #29663

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, GFP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTP1112

    Plasmid

    #104158

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLifeAct-mTurquoise2

    Plasmid

    #36201

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    LifeAct
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5B-HA-Smad2

    Plasmid

    #11734

    Depositor

    Jeff Wrana

    Article

    Eppert et al Cell. 1996 Aug 23. 86(4):543-52.

    Insert

    SMAD2 (SMAD2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 3, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCI HA NEDD4

    Plasmid

    #27002

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4 (NEDD4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. mCherry-Lysosomes-20

    Plasmid

    #55073

    Purpose

    Localization: Lysosome Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-ABE7.10

    Plasmid

    #102919

    Purpose

    expresses ABE7.10 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.10
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. phage-cmv-cfp-24xpp7

    Plasmid

    #40652

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    CFP-24xPP7
    

    Use

    Lentiviral

    Tags

    CFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. cyto-Ruby3-iATPSnFR1.0

    Plasmid

    #102551

    Purpose

    expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    Ruby3-iATPSnFR1.0
    

    Tags

    Ruby3

    Expression

    Mammalian

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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