8,812 results

1. pROSA26-PA

   Plasmid

   #21271

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Available Since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dronpa3-N1

   Plasmid

   #54682

   Purpose

   Localization: N1 Cloning Vector, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   Dronpa3

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. tdTomato-N1

   Plasmid

   #54642

   Purpose

   Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson, Nathan Shaner, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. hFerritinH

   Plasmid

   #122652

   Purpose

   Expression of hFerritinH in bacterial cells

   Depositor

   Trevor Douglas

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Expression

   Bacterial

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FH-TET1-pEF

   Plasmid

   #49792

   Purpose

   This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

   Depositor

   Anjana Rao

   Article

   Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

   Insert

   Tet methylcytosine dioxygenase 1 (TET1 Human)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-Traditional CAR (anti-CD19)

   Plasmid

   #192115

   Purpose

   Lentiviral delivery of Traditional CAR(anti-CD19)

   Depositor

   Wilson Wong

   Article

   Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

   Insert

   pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
   

   Use

   Lentiviral

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFPGUSPlus

   Plasmid

   #64401

   Purpose

   binary plant vector expressing GFP and GUS, both driven by a 35S promoter

   Depositor

   Claudia Vickers

   Article

   Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

   Inserts

   GFP
   GUSPlus
   

   Use

   Binary vector for plant transformation

   Tags

   His

   Expression

   Plant

   Mutation

   S65T

   Promoter

   35S

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCT5-bac2.0

   Plasmid

   #119872

   Purpose

   Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

   Depositor

   Claudia Schmidt-dannert

   Article

   Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

   Inserts

   CymR
   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pveg promoter with CuO sequence and PxylR promoter

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. LZ10 PBREBAC-H2BHalo

    Plasmid

    #91564

    Purpose

    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

    Depositor

    James Zhe Liu

    Article

    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

    Insert

    histone H2B-HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

    Plasmid

    #153004

    Purpose

    Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (N) (G78)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1-HyPerRed-mito

    Plasmid

    #60247

    Purpose

    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HyPerRed-mito
    

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SeeSaw Reporter (SSR2.0)

    Plasmid

    #50393

    Purpose

    SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

    Depositor

    Pablo Huertas Sánchez

    Article

    Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

    Insert

    SSR2.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PTEN

    Plasmid

    #28298

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE-TIGHT-EGFP-donor fw copy

    Plasmid

    #22074

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2/HB

    Plasmid

    #26557

    Depositor

    Perry Hackett

    Insert

    pT2/HB
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-TLR9-YFP

    Plasmid

    #13642

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 9 (TLR9 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C3-Sec5

    Plasmid

    #53756

    Purpose

    pEGFP-C3 plasmid that expresses Sec5

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec5 (EXOC2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only