We narrowed to 13,645 results for: NTS

1. pHAGE2-TetO-GFP-mSox17

   Plasmid

   #206373

   Purpose

   Expresses EGFP fused mouse SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSR

   Plasmid

   #161983

   Purpose

   Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

   Depositor

   Olga Soutourina

   Article

   Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMP71-Flt3L

   Plasmid

   #174600

   Purpose

   Secretes the extracellular domain of murine Flt3L

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   secretes extracellular domain of mouse Flt3-L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEE391

   Plasmid

   #149281

   Purpose

   T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA_FT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOD_A1110

   Plasmid

   #91031

   Purpose

   Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ZmUbi

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Erk2

   Plasmid

   #29582

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   HIS6 and TEV

   Expression

   Bacterial

   Mutation

   Starting Met removed from Erk2

   Promoter

   Available since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU55

   Plasmid

   #167684

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::asCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMOD_A0103

   Plasmid

   #91000

   Purpose

   Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYU-2-Env

   Plasmid

   #133997

   Purpose

   Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

   Depositor

   Himanshu Garg

   Article

   Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

   Insert

   Envelope Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. gRNAy

    Plasmid

    #168294

    Purpose

    Expresses gRNA targeting the yellow gene in Drosophila

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    U6.3-gRNA[y]-scaffold
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UBQ10:sXVE:(MCS)-S11

    Plasmid

    #108179

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. miR34a in pcDNA3.1

    Plasmid

    #78125

    Purpose

    Mammalian expression of miR34

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    miR34 (MIR34A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBac{3XP3::DsRed, Pactin::Ptrsps}

    Plasmid

    #86862

    Purpose

    Expresses DsRed in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S129A

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Stx1a-12aa-mCer

    Plasmid

    #53236

    Purpose

    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    Syntaxin 1A (Stx1a Rat)
    

    Use

    Tags

    12aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pTRANS_211

    Plasmid

    #91110

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p2T-CMV-eA3Amax-BlastR

    Plasmid

    #152997

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hSyn1-tdTomatof

    Plasmid

    #104060

    Purpose

    Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCTCON2-BYG

    Plasmid

    #158126

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    mTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PKG I beta WT

    Plasmid

    #16394

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 beta (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211843

    Purpose

    Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMOD_A-CmYLCV-dCas9-24XGP41

    Plasmid

    #202009

    Purpose

    Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GS linkers

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDGE6

    Plasmid

    #79476

    Purpose

    sgRNA shuttle vector; module 1E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCBC-MT3T4

    Plasmid

    #50595

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-26t plus, OsU3p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIRES2-EGFP Rac2

    Plasmid

    #12193

    Depositor

    Gary Johnson

    Article

    Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

    Insert

    Rac2 (RAC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-tag2B-PISP/AIPP1

    Plasmid

    #47606

    Depositor

    Emanuel Strehler

    Article

    Goellner et al Ann N Y Acad Sci. 2003 Apr;986:461-71.

    Insert

    PISP (PDZD11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pOPS0377

    Plasmid

    #115509

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-flex-oChIEF-citrine

    Plasmid

    #50973

    Purpose

    flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    inverted and inserted between two lox sites

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Hygro-iDEST

    Plasmid

    #75339

    Purpose

    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    TRE-DEST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Jnk3a1

    Plasmid

    #29577

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Jnk3a1 (MAPK10 Human)
    

    Use

    Tags

    HIS6 and TEV

    Expression

    Bacterial

    Mutation

    Contains residues 40-402

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pJBL727

    Plasmid

    #140397

    Purpose

    Expresses CsoR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CsoR-T
    

    Use

    Tags

    6XHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE-UBC-MCP-mNeonGreen

    Plasmid

    #207581

    Purpose

    The lentiviral plasmids for expression of MCP-NeonGreen

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    MS2 coat protein mNeonGreen fusion
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    UBC

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FSW-HRP-V5-NLGN2

    Plasmid

    #82545

    Purpose

    Overexpression vector for V5-NLGN2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    NLGN2 (Nlgn2 Rat)
    

    Use

    Lentiviral

    Tags

    HRP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FSW-HRP-V5-SLITRK3

    Plasmid

    #82539

    Purpose

    Lentiviral vector for expressing HRP-SLITRK3 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    SLITRK3 (SLITRK3 Human)
    

    Use

    Lentiviral

    Tags

    HRP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBJ7

    Plasmid

    #167136

    Purpose

    A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEYFP-C3-HA-Ubiquitin

    Plasmid

    #28236

    Depositor

    Michael Mancini

    Article

    Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

    Insert

    Ubiquitin (UBB Human)
    

    Use

    Tags

    EYFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. XMRV vp62

    Plasmid

    #26189

    Depositor

    Joseph DeRisi

    Article

    Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

    Insert

    XMRV VP62 (gag Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pTRANS_213

    Plasmid

    #91112

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmNFH

    Plasmid

    #85178

    Purpose

    Mouse neurofilament protein H cDNA mammalian expression construct

    Depositor

    Anthony Brown

    Article

    Colakoglu et al J Cell Biol. 2009 Jun 1;185(5):769-77. d

    Insert

    mouse NFH (Nefh Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. A3A-Gam

    Plasmid

    #119769

    Purpose

    Conversion of C to T monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge