165,947 results

1. pSBBi-Pur GFP-TM

   Plasmid

   #139185

   Purpose

   SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.

   Depositor

   Neal Devaraj

   Insert

   GFP-TM
   

   Use

   Transposon

   Expression

   Mammalian

   Mutation

   fused to membrane anchor

   Promoter

   EF1α

   Available Since

   March 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP573

   Plasmid

   #98796

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-ENLYFQS-TEV WT
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-His6-FnCas9GFP

   Plasmid

   #130966

   Purpose

   expression of FnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   HA-NLS-FnCas9
   

   Use

   CRISPR

   Tags

   6xHis-mEGFP-TEV-HA-NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-6xHis-GFP(-30)

   Plasmid

   #199167

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(-30)
   

   Tags

   MGHHHHHHGGA

   Mutation

   T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 Flag Erg

   Plasmid

   #66977

   Purpose

   Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Erg (Erg Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035)

   Plasmid

   #214853

   Purpose

   Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs)

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   CoChR-EGFP
   

   Use

   AAV

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF3427

   Plasmid

   #144903

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFIB (NFIB Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18T-mini-Tn7T-hph-Ptet2

   Plasmid

   #218368

   Purpose

   Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CMV-jGCaMP8f

   Plasmid

   #162373

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLX1.8MitoKaede

    Plasmid

    #28133

    Depositor

    Geoffrey Owens

    Insert

    MitoKaede
    

    Use

    Lentiviral

    Tags

    COX VIII signal sequence

    Expression

    Mammalian

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221_SLC30A5

    Plasmid

    #132077

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A5 (SLC30A5 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pBLADE(FP6*)-mCherry

    Plasmid

    #168048

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Inserts

    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    

    Expression

    Bacterial

    Promoter

    J23101* and PBAD

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRC/CMV-JAK1-VSV

    Plasmid

    #139356

    Purpose

    Expression of JAK1 protein

    Depositor

    Sandra Pellegrini

    Article

    Richter et al J Biol Chem. 1998 Sep 18;273(38):24723-9

    Insert

    JAK1 cDNA with 300nt of 5'UTR (JAK1 Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xmNeonGreen_UtrCH

    Plasmid

    #129607

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Utrophin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-C-mNeon-Blast

    Plasmid

    #207709

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0093

    Plasmid

    #143009

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTCF37 HLA-B*35:01

    Plasmid

    #180450

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*35:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-RNF126

    Plasmid

    #138647

    Purpose

    Mammalian expression of 2HA-tagged RNF126

    Depositor

    Jane McGlade

    Article

    Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

    Insert

    RNF126 (RNF126 Mouse)
    

    Tags

    HA-HA

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLv-EF1a-Parkin-A92mKO2

    Plasmid

    #213559

    Purpose

    Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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