161,439 results

1. pFRT/TO/FLAG/HA-DDX54

   Plasmid

   #97060

   Purpose

   Expresses FLAG/HA-tagged DDX54 in mammalian cells

   Depositor

   Markus Landthaler

   Article

   Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

   Insert

   DDX54 (DDX54 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

2. GFP-GLT1a

   Plasmid

   #162515

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1a (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EF1a-jGCaMP8m-WPRE

   Plasmid

   #176757

   Purpose

   AAV-mediated expression of GCaMP8m under the EF1a promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_FBXW7_p.R465C

   Plasmid

   #81499

   Purpose

   Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FBXW7 (FBXW7 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   R465C

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

   Plasmid

   #61629

   Purpose

   R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   mDII
   ntdTomato
   DII
   n3xVenus
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEYFP-Synectin

   Plasmid

   #35791

   Depositor

   Arie Horowitz

   Article

   Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

   Insert

   Synectin (Gipc1 Mouse)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   K111R

   Promoter

   CMV

   Available since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

8. EF1a-MVPH

   Plasmid

   #235594

   Purpose

   Expresses the MS2-VP64-P65-HSF1 under the control of human EF1a promoter

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   MS2-VP64-P65-HSF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PTGS1-bio-His

   Plasmid

   #53414

   Purpose

   Expresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PTGS1 (PTGS1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132410

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dEsCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R295A; H300A; R849A; H854A

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 560HTR

    Plasmid

    #24444

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pRS428

    Plasmid

    #11257

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. 12x PRE TK luc

    Plasmid

    #206163

    Purpose

    Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    12X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR223-GFP

    Plasmid

    #198589

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    GFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

    Plasmid

    #156407

    Purpose

    pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    mutant KRAS4B-G12V

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-CAG-3xNLS-AausFP1

    Plasmid

    #191096

    Purpose

    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-AausFP1
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV enhancer and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRF2ΔBΔM/pENTR

    Plasmid

    #19797

    Depositor

    Mark Mattson

    Article

    van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

    Insert

    TRF2 (TERF2 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    deleted amino acids 1-44 and 450-500

    Promoter

    Available since

    April 15, 2009

    Availability

    Academic Institutions and Nonprofits only

18. LAMP1-SBP-GFP

    Plasmid

    #120172

    Purpose

    Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Lysosomal-associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV4 p50

    Plasmid

    #21965

    Depositor

    Warner Greene

    Article

    Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

    Insert

    NF-kB p50 subunit (NFKB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-PTK7DN-VSV

    Plasmid

    #65251

    Purpose

    Expression of VSV tagged dominant negative PTK7 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

    Insert

    PTK7DN (PTK7 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    deleted AA 737-1071

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only