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161,838 results

Showing: 3421 - 3450 of 161838 results

  9. CMV-mito-GEM-GECO1

    Plasmid
    #32461
    Purpose
    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32461-plasmid-map-sequence-id-123705

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  10. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

    Plasmid
    #196227
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn iGluSnFR3 v857.NGR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and NGR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196227-plasmid-map-sequence-id-409161

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  12. pHN781

    Plasmid
    #178828
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178828-plasmid-map-sequence-id-351929

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  14. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  15. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid
    #184780
    Purpose
    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Activating tyrosine kinase domain mutation (D835Y)
    Promoter
    MSCV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    184780-plasmid-map-sequence-id-367916

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  17. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

    Plasmid
    #216665
    Purpose
    Expresses Gal4 inducible BFP, with hygromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    BFP
    HygromycinR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216665-plasmid-map-sequence-id-432189

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  19. pAAV-hSyn-EGFP

    Plasmid
    #50465
    Purpose
    EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50465-plasmid-map-sequence-id-240907

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  20. TFORF1847

    Plasmid
    #142868
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ETS1 (ETS1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    142868-plasmid-map-sequence-id-385800

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  21. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid
    #80921
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80921-plasmid-map-sequence-id-234619

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  23. 458 p5E-uasE1b

    Plasmid
    #26026
    Depositor
    Nathan Lawson
    Insert
    E1B basal promoter + UAS repeats
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    26026-plasmid-map-sequence-id-12211

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  25. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

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  27. pSBbi-RP

    Plasmid
    #60513
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60513-plasmid-map-sequence-id-373029

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Showing: 3421 - 3450 of 161838 results