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Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorInsertVEGFA (Vegfa Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS2
Plasmid#196981PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS2 tags
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS1
Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT3A
Plasmid#35521PurposeMammalian expression of human DNMT3A with myc tagDepositorInsertDNMT3A (DNMT3A Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-1
Plasmid#208790PurposeInducibly expresses human Caspase1, fused to light-activatable Cry2DepositorInsertCaspase1 (CASP1 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadDE
Plasmid#193837PurposeExpress TadDE (with SpCas9) in mammalian cellsDepositorInsertTadA-DualEditor-Cas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhyg-H3.3WT-FLAG/HA
Plasmid#128740PurposeExpression of histone H3.3WT-FLAG/HADepositorInsertH3.3 WT (H3-3A Human)
UseRetroviralTagsFLAG and HAExpressionMutationPromoterMSCVAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhyg-H3.3K4M-FLAG/HA
Plasmid#128741PurposeExpression of histone H3.3K4M-FLAG/HADepositorInsertH3.3K4M (H3-3A Human)
UseRetroviralTagsFLAG and HAExpressionMutationK4MPromoterMSCVAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0
Plasmid#164606PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterhSynAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6XHis-human SUMO 1
Plasmid#133770Purposeexpress 6Xhis- SUMO 1 proteinDepositorInsertSUMO 1 (SUMO1 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
YEE-BE4max
Plasmid#138157PurposeC-to-T base editorDepositorInsertrAPOBEC1(YEE)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E + R132E; within Cas9…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor
Plasmid#184951PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RhoA Biosensor
Plasmid#68026PurposeC-terminus of Anillin in mammalian expression vector pEGFP-C1DepositorInsertAnillin (AHD+PH) (ANLN Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
UseTagsExpressionBacterialMutationPromoterglnS and pBADAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-TadCBEd-ivt-template
Plasmid#193843PurposeTemplate for in vitro transcription of TadCBEd (with SpCas9)DepositorInsertTadCBEd with SpCas9
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-TagBFP
Plasmid#120910PurposeExpresses BFP tagged Ptch1-BDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHA and TagBFPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AALN-BE4max
Plasmid#138161PurposeC-to-T base editorDepositorInsertrAPOBEC1(AALN)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 R33A + K34A + H122L + D124N; with…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX
Plasmid#164611PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in mammalian cellsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseTagsExpressionMammalianMutationS383TPromoterCMVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX
Plasmid#164612PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cellsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseTagsExpressionMammalianMutationS383T, F177APromoterCMVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6**)-mCherry
Plasmid#168049PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101** and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
A&C-BEmax
Plasmid#157947PurposeMammalian expression of A&C-BEmaxDepositorInsertA&C-BEmax
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-MDA5-Full-length
Plasmid#210826Purposeprotein expression in ecoliDepositorInsertMDA5 (IFIH1) Full Length Protein (IFIH1 Human)
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB1679 CMV-HIVNES-GS-Cas13bt3
Plasmid#176317PurposeCMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)DepositorInserthuman codon optimized Cas13bt3
UseCRISPRTagsHIV NESExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB1678 CMV-HIVNES-GS-Cas13bt1
Plasmid#176316PurposeCMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)DepositorInserthuman codon optimized Cas13bt1
UseCRISPRTagsHIV NESExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct3
Plasmid#28196DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa170-414 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_TAF3_2xPhd
Plasmid#179399PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhdDepositorInsertTAF3_2xPhd
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-AncBE4max
Plasmid#174164PurposeThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.DepositorInsertAncBE4max
UseLentiviralTagsP2A-mCherryExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6*)-mCherry
Plasmid#168048PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101* and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_2xChromo
Plasmid#179396PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromoDepositorInsertCBX7_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationG113V in flexible linkerPromoterCAGGSAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only