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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,828 results for: 158

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Showing: 3421 - 3450 of 10828 results

  2. pJB02

    Plasmid
    #111959
    Purpose
    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    T7
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111959-plasmid-map-sequence-id-219913

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  4. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid
    #191154
    Purpose
    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    EGFP-t70587
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV-TO
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191154-plasmid-map-sequence-id-383934

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Showing: 3421 - 3450 of 10828 results