We narrowed to 10,943 results for: NSI;

1. pMC7-XI3dw

   Plasmid

   #176171

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMC8b-XI5up

   Plasmid

   #176172

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XI-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC7-XI5dw

   Plasmid

   #176173

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMC8b-XII5up

   Plasmid

   #176176

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XII-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMC7-XII5dw

   Plasmid

   #176177

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XII-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC8b-X2up

   Plasmid

   #176162

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site X-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMC7-X2dw

   Plasmid

   #176163

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site X-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

   Plasmid

   #72240

   Purpose

   Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   GLT1 GAL1p 24xPP7 V3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Flag-YAP-del 50-59

   Plasmid

   #33065

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 50-59

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pSW179 - pML1

    Plasmid

    #115977

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pML1
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL-p27UTR

    Plasmid

    #20881

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p27 3'UTR (Cdkn1b Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pJW4.21

    Plasmid

    #62875

    Purpose

    Bacterial expression of MBP-MEG-1

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-MEG-1
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-GATA2-DCzf

    Plasmid

    #1292

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcihygrosynDAT

    Plasmid

    #19992

    Depositor

    Jonathan Javitch

    Article

    Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

    Insert

    synDAT (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p6721 MSCV-IP N FlagHA 16E6 8S9A10T

    Plasmid

    #44153

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    E6 8S9A10T mutant

    Promoter

    MSCV LTR

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

16. p6722 MSCV-IP N FlagHA 16E6 I128T

    Plasmid

    #44154

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    HPV16 E6 I128T mutant

    Promoter

    MSCV LTR

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Use

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PI(LM)-Rac1(WT)dc1.FLARE

    Plasmid

    #87590

    Purpose

    PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    PBD
    Rac1
    

    Use

    Tags

    YPet and dTurq

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PmarA-cfp

    Plasmid

    #79867

    Purpose

    Degradation tagged CFP fluorescent reporter for MarA

    Depositor

    Mary Dunlop

    Article

    El_Meouche et al Sci Rep. 2016 Jan 13;6:19538. doi: 10.10

    Insert

    A modified marRAB promoter upstream of a degradation tagged cfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    modified marRAB promoter with inactivated MarR bi…

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP Snail 4SE

    Plasmid

    #16230

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    4SE: S108E, S112E, S116E, S120E

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pDG279

    Plasmid

    #85219

    Purpose

    mammalian expression of ubiquitin-K48R-EGFP fusion

    Depositor

    Douglas Gray

    Article

    Tsirigotis et al J Biol Chem. 2001 Dec 7;276(49):46073-8.

    Insert

    ubiquitin C (UBC Human)
    

    Use

    Tags

    6xHIS and EGFP

    Expression

    Mammalian

    Mutation

    K48R

    Promoter

    human ubiquitin C

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

22. SERA7-bio-His

    Plasmid

    #50819

    Purpose

    Expresses enzymatically monobiotinylated full length SERA7

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA7
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

    Plasmid

    #140087

    Purpose

    Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    LbCpf1 (LbCas12a) crRNA backbone
    

    Use

    Lentiviral

    Tags

    EF1a-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAB029f

    Plasmid

    #79220

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ lambda cI-SH2ABL1; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ; PlacIq

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TU#1450_unc-53a_cDNA

    Plasmid

    #82361

    Purpose

    express unc-53a cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-53 a isoform (unc-53 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-TRE-DIO-SwiChR-eYFP

    Plasmid

    #166604

    Purpose

    Encodes Cre-dependent SwiChR-eYFP under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    SwiChR-eYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LacD.1_WT_pET21a

    Plasmid

    #29789

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

28. LacD_L165E/L275S_pET21a

    Plasmid

    #29788

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L165E and L275S

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pBT241_(pCA-ATG-intron-tTA2)

    Plasmid

    #36875

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tTA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAB031a

    Plasmid

    #79221

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ HA4-Zif268; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ; PlacIq

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Dom34+GST

    Plasmid

    #19099

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag, GST, and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

32. MAC_C_PLRKT

    Plasmid

    #111673

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PLRKT (PLGRKT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Ta1098

    Plasmid

    #19072

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

34. Tip alpha (21-192)

    Plasmid

    #23157

    Depositor

    SeWon Suh

    Article

    Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

    Insert

    TNF-α-inducing protein
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-20

    Promoter

    Available since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pGL-RbUTR

    Plasmid

    #20883

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    Rb 3'UTR (Rb1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

36. AAV-CMV-NLS-mTurquoise2-optoNES-WPRE

    Plasmid

    #159962

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28-his-3C_Clu3B6p

    Plasmid

    #100093

    Purpose

    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

    Insert

    14-3-3 sigma human (SFN Human)
    

    Use

    Tags

    HSPB6 peptide and His6

    Expression

    Bacterial

    Mutation

    75-77 residues changed to AAA to facilitate cryst…

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SERA6-bio-His

    Plasmid

    #50818

    Purpose

    Expresses enzymatically monobiotinylated full length SERA6

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA6
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MAC_N_RS6

    Plasmid

    #111694

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    RS6 (RPS6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Cdc42 T17N pCS2

    Plasmid

    #16670

    Depositor

    De-Li Shi

    Article

    Djiane et al Development. 2000 Jul . 127(14):3091-100

    Insert

    Cdc42 T17N (CDC42 Human)
    

    Use

    Vertebrate

    Tags

    Expression

    Mutation

    T17N. Dominant negative.

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only