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We narrowed to 7,002 results for: tet

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Showing: 5701 - 5750 of 7002 results

  5. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119190-plasmid-map-sequence-id-232293

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  6. circRAJ31-Toehold_mutant

    Plasmid
    #69930
    Purpose
    Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.
    Depositor
    Alfonso Jaramillo
    Article
    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do
    Insert
    plTet-circRAJ31_toeholdmutant
    Use
    Synthetic Biology
    Mutation
    4 bases mutated in toehold of riboregulator
    Promoter
    PltetO1-2
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    69930-plasmid-map-sequence-id-124217

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  7. pYCTK028

    Plasmid
    #176732
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    ste2Tp
    Use
    Synthetic Biology
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176732-plasmid-map-sequence-id-351572

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  11. pTS2163

    Plasmid
    #169632
    Purpose
    Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
    Expression
    Mammalian
    Promoter
    tetO7-PhCMVmin / PPGK
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169632-plasmid-map-sequence-id-366785

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  12. JA431

    Plasmid
    #49937
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD KL-2 (KLF4 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. JA1290

    Plasmid
    #49968
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-9 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. JA1261

    Plasmid
    #49963
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. JA1252

    Plasmid
    #49956
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-3 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. MLM3733

    Plasmid
    #49964
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. Equalizer-H plasmid with onboard mCherry cassette

    Plasmid
    #169733
    Purpose
    Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169733-plasmid-map-sequence-id-334140

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  18. pDUP1

    Plasmid
    #223516
    Purpose
    inactive cogfp under control of Ptet, cogfp under control of Ptac
    Depositor
    Yolanda Schaerli
    Article
    Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75
    Inserts
    CoGFP
    CoGFP inactivated
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    anti-dimerization mutations S129G, 22 C154S, D156…
    Promoter
    Ptac, Ptac and Ptet, and Ptet
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    223516-plasmid-map-sequence-id-444180

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  22. Equalizer-L plasmid with onboard mCherry cassette

    Plasmid
    #169735
    Purpose
    Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169735-plasmid-map-sequence-id-334142

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  25. TRAP 4-mEOS

    Plasmid
    #74127
    Purpose
    Expresses TRAP 4 fused to mEOS3.2
    Depositor
    Lynne Regan
    Insert
    TRAP 4 fused to mEOS3.2
    Expression
    Bacterial
    Promoter
    PLTETO
    Available Since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74127-plasmid-map-sequence-id-148240

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  28. pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

    Plasmid
    #69586
    Purpose
    Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus
    Depositor
    Galit Lahav
    Article
    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:
    Insert
    p53-L344P (TP53 Human)
    Use
    Lentiviral
    Tags
    mKate2 and split mVenus - N term
    Expression
    Mammalian
    Mutation
    p53 L344P mutation that prevents dimerization and…
    Promoter
    EF1alpha
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    69586-plasmid-map-sequence-id-135228

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  32. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Tags
    3x-FLAG
    Expression
    Bacterial
    Promoter
    pTet
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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  35. PTS

    Plasmid
    #39015
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTS (PTS Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  46. LT3GEPIR-WEE1shRNA1

    Plasmid
    #220571
    Purpose
    To inducibly knockdown WEE1 expression
    Depositor
    Sean Lee
    Insert
    WEE1 shRNA1 (WEE1 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220571-plasmid-map-sequence-id-438397

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  47. LT3GEPIR-WEE1shRNA2

    Plasmid
    #220572
    Purpose
    To inducibly knockdown WEE1 expression
    Depositor
    Sean Lee
    Insert
    WEE1 shRNA2 (WEE1 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220572-plasmid-map-sequence-id-437939

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Showing: 5701 - 5750 of 7002 results