We narrowed to 4,947 results for: mcs

1. pmCherry C1 Omp25

   Plasmid

   #157758

   Purpose

   Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   SYNJ2bp (SYNJ2BP Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194381

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194693

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

   Plasmid

   #194694

   Purpose

   CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194695

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-IP- SCAT3.2

   Plasmid

   #172497

   Purpose

   SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Amcyan
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   MLV LTR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-p53DD-pcDNA3

   Plasmid

   #25989

   Depositor

   William Kaelin

   Article

   Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

   Insert

   tumor protein p53 (TP53 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   truncated cDNA encodes truncated protein (aa 300-…

   Available Since

   Oct. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. IL1RN gRNA1

   Plasmid

   #113130

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6 for gRNA expression, RSV for GFP expression

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330 PLIN2

   Plasmid

   #202196

   Purpose

   pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4.TO-ORF8-2xCSTREP

    Plasmid

    #136172

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF8

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF8
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4.TO-K8.1-2xCSTREP

    Plasmid

    #136157

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8.1

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K8.1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry N1 MFN2

    Plasmid

    #157759

    Purpose

    Expression of MFN2:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    MFN2 (MFN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized

    Plasmid

    #172345

    Purpose

    Expression of FLAG-tagged, codon-optimized, full-length SRSF3

    Depositor

    Sujatha Jagannathan

    Article

    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

    Insert

    serine and arginine rich splicing factor 3 (SRSF3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    EF-1a

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT301 BxBi Lenti pEF-attB LSR GFP

    Plasmid

    #184944

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Patrick Hsu, Michael Bassik, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    BxBi Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-Tet-On-shRab27a

    Plasmid

    #120930

    Purpose

    conditionally knockdown Rab27a in mouse cell lines

    Depositor

    Mien-Chie Hung

    Article

    Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

    Insert

    shRab27a (Rab27a Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

    Plasmid

    #194279

    Purpose

    Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized VP64 dSaCas9 VP64 T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hU6 and hUbC

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Integrin beta5-2XEGFP

    Plasmid

    #139779

    Purpose

    Detecting Integrin beta5 by fluorescence microscopy

    Depositor

    Staffan Stromblad

    Article

    Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

    Insert

    Integrin subunit beta5 (ITGB5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

    Plasmid

    #44027

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P405A, P530V, N851A

    Promoter

    CMV

    Available Since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-PKM2-R399E

    Plasmid

    #122691

    Purpose

    Express His-tagged PKM2 (R399E) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    R399E

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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