20,043 results

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LentiCRISPRv2-Opti

Plasmid

#163126

Purpose

LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-mPD-L1

Plasmid

#121488

Purpose

Expression of mouse PD-L1 WT

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphai1-RLuc8

Plasmid

#140973

Purpose

Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphai1-RLuc8 (GNAI1 Human)

Use

Synthetic Biology

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pG12-SEAP

Plasmid

#170271

Purpose

Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

SEAP

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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3X ERE TATA luc

Plasmid

#11354

Purpose

Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ERE

Use

Luciferase

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCPRDEnv

Plasmid

#1732

Purpose

This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

Depositor

Garry Nolan

Insert

Gag-Pol and Rev

Expression

Mammalian

Available Since

Oct. 24, 2005

Availability

Academic Institutions and Nonprofits only

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ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

Plasmid

#85039

Purpose

For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

RFP-P2A-tTA

Use

AAV

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET16B.Pfu

Plasmid

#12509

Purpose

Bacterial expression of Pfu DNA polymerase

Depositor

Sung-Hou Kim

Article

Goldman et al Acta Crystallogr D Biol Crystallogr. 199

Insert

Pfu DNA polymerase

Expression

Bacterial

Mutation

Y218H, Q462R, F533L, K559I and V683A (mutations w…

Available Since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SHP2 WT

Plasmid

#8381

Depositor

Ben Neel

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO mCherry

Plasmid

#114471

Purpose

Flp-dependent mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

Ef1a

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK7

Plasmid

#23395

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK7 (NEK7 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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35S-eGFP-nosT

Plasmid

#80127

Purpose

Transient expression vector for eGFP in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Type

Empty backbone

Tags

eGFP

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD33.1

Plasmid

#36267

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Type

Empty backbone

Expression

Bacterial

Promoter

PBAD

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV1, AAV5, and AAV8

Inserts

Caspase 3 (CASP3 Human, Synthetic)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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