8,812 results

1. AAVS1-SA-2A-NEO-CAG-RTTA3

   Plasmid

   #60431

   Purpose

   neomycin resistance; constitutive promoter driving reverse tet activator

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   rtTA3
   

   Use

   Zfn donor

   Promoter

   CAG

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

   Plasmid

   #39299

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-DDB1

   Plasmid

   #19918

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Akt1(WT)

   Plasmid

   #86637

   Purpose

   Expresses eGFP-tagged human Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Raptor_2 shRNA

   Plasmid

   #1858

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TRE-dCas9-KRAB-MeCP2

   Plasmid

   #122267

   Purpose

   SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Tags

   dCas9-KRAB-MeCP2

   Expression

   Mammalian

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a-BirA-V5-neo

   Plasmid

   #100548

   Purpose

   Expresses C-terminal V5-tagged BirA biotin ligase

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   C-terminal V5-tagged BirA
   

   Tags

   V5, His

   Expression

   Mammalian

   Promoter

   Human EF1a

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKDsgRNA-p15

   Plasmid

   #62656

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-CXCL12-sfGFP

   Plasmid

   #98961

   Purpose

   Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCL12 (CXCL12 Human)
   

   Tags

   Superfolder GFP

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCVD442

    Plasmid

    #11074

    Depositor

    James Kaper, Michael Donnenberg

    Article

    Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

    Insert

    sacB (sacB Bacillus subtilis)
    

    Expression

    Bacterial

    Available Since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    His and roGFP2

    Expression

    Bacterial

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_TRE3G-p300-dCas9-P2A-mCherry

    Plasmid

    #138456

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    p300 core
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TetO-FUW-OSKM

    Plasmid

    #20321

    Purpose

    Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_FBXW7_WT

    Plasmid

    #81795

    Purpose

    Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FBXW7 (FBXW7 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI-EGFP-NR2a wt

    Plasmid

    #45445

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2A (Grin2a Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetON-shYAP

    Plasmid

    #115667

    Purpose

    Inducible YAP knockdown

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP shRNA (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBV-Luc

    Plasmid

    #16539

    Purpose

    luciferase reporter plasmid with very low basal activity

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 3xFlag IFIT1

    Plasmid

    #53554

    Purpose

    mammalian expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b His6-FKBPF36V

    Plasmid

    #73180

    Purpose

    Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

    Depositor

    Thomas Wandless

    Article

    Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

    Insert

    FKBP(F36V) (FKBP1A Human)
    

    Tags

    His 6x

    Expression

    Bacterial

    Mutation

    F36V

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. mycBioID2-13X Linker-MCS

    Plasmid

    #92308

    Purpose

    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Humanized A. aeolicus BioID2 with R40G and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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