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pLVpuro-CMV-N-3xFLAG

Plasmid

#123223

Purpose

N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFC332

Plasmid

#87845

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
hph (hygromycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSG5-HA-p300

Plasmid

#89094

Purpose

mammalian expression of full-length human p300 in pSG5

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMIR-FLAG-MLL-AF9

Plasmid

#71444

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

Depositor

Daisuke Nakada

Publication

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-AF9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS6-YFP-SMASh

Plasmid

#68853

Purpose

Expresses SMASh tag fused to YFP at C terminus in mammalian cells

Depositor

Michael Lin

Publication

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP-SMASh

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Publication

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2C Promoter (2-cell stage promoter)

Tags

tdTomato

Expression

Mammalian

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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Ac5-STABLE1-neo

Plasmid

#32425

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

GFP-T2A-neo

Expression

Insect

Promoter

Actin5c

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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myc-hULK1

Plasmid

#31961

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ULK1 (ULK1 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAc-Neo-OVA

Plasmid

#22533

Depositor

Michael Bevan

Publication

Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Available since

Dec. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL2Basic-EcadK1

Plasmid

#19290

Depositor

Eric Fearon

Publication

Hajra et al Oncogene. 1999 Dec 2. 18(51):7274-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMIG-hOCT4

Plasmid

#17225

Depositor

George Daley

Publication

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

POU class 5 homeobox 1 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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HA-CYLD-WT

Plasmid

#15506

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

CYLD (CYLD Human)

Tags

HA

Expression

Mammalian

Available since

Aug. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Publication

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCMV-SynA

Plasmid

#174860

Purpose

Expresses mouse syncytin A in mammalian cells

Depositor

Feng Zhang

Publication

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin A (Syna Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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