12,610 results

1. pGEX-2T-GST-eIF4E K119A

   Plasmid

   #112818

   Purpose

   Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Eif4e (Eif4e Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K119 mutated to A, increasing affinity for cap st…

   Promoter

   T7

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD40L-MIEG-hCD4 (hCD4-CD40L)

   Plasmid

   #40355

   Depositor

   Alexander Dent

   Article

   Kusam et al Int J Cancer. 2009 Aug 15;125(4):977-81.

   Insert

   CD40L (Cd40lg Mouse)
   

   Use

   Retroviral

   Tags

   IRES hCD4

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET23a-mUbc9

   Plasmid

   #53137

   Purpose

   E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Ubc9 (Ube2i Mouse)
   

   Use

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   protein sequence is identical to human

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-hygro-2xMyc-Rac1-WT

   Plasmid

   #128580

   Purpose

   For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCytERM-BDFP1.8-N1

   Plasmid

   #197186

   Purpose

   Expresses the fusion protein of CytERM-BDFP1.8 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   CytERM-BDFP1.8-
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH-nCas9-PPE

   Plasmid

   #140447

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1, OsU3

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sema6a.a-AP-His

   Plasmid

   #72037

   Purpose

   Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6a.a (Sema6a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-Foxo1-WT 6KQ

   Plasmid

   #17562

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1 6KQ (Foxo1 Mouse)
   

   Use

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   K242, K245, K259, K262, K271 and K291 were replac…

   Promoter

   CMV

   Available since

   Oct. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pCIG2-Flag-mNuak1 WT

   Plasmid

   #168507

   Purpose

   For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   Nuak1 (Nuak1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn-DIO-mCReP

    Plasmid

    #167503

    Purpose

    Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    Ppp1r15b (Ppp1r15b Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-Cluc-RMA

    Plasmid

    #189624

    Purpose

    Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    Cypridina luciferase fused to Fc
    

    Use

    AAV and Luciferase

    Tags

    Cluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMX IkB alpha M

    Plasmid

    #12329

    Depositor

    Inder Verma

    Article

    Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

    Insert

    IkB alpha M (Nfkbia Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dominant-negative mutant. Many signal transductio…

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN716 - pCAGGS-eGFP-3Xnls-bpA

    Plasmid

    #156435

    Purpose

    For transient expression of nuclear eGFP in mammalian cells

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-emiRFP670-P2A-GFP

    Plasmid

    #197160

    Purpose

    Expresses the protein of emiRFP670-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    emiRFP670-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB700-mouse-ACTC1-Puro

    Plasmid

    #128326

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Flag-Kdm6b(1025-End)-pA

    Plasmid

    #100278

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    catalytic domain (1025aa-End)

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xO/S luc

    Plasmid

    #69445

    Purpose

    detection/assessment of OCT4 and SOX2 transcription factor activity

    Depositor

    Lisa Dailey

    Insert

    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUW-tetO-Irf1

    Plasmid

    #139833

    Purpose

    doxycycline-inducible expression of mouse Irf1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf1 (Irf1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Nanog-2A-GFP

    Plasmid

    #59994

    Purpose

    Inserts GFP immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIEG3-JunB

    Plasmid

    #40349

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    JunB (Jun Mouse)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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