165,343 results

1. TFORF2894

   Plasmid

   #144190

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MTA1 (MTA1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ND6-L1397-N_UGI-NES_right

   Plasmid

   #187846

   Purpose

   expresses the right-side half ND6-UGI-NES in mammalian cells

   Depositor

   Chengqi Yi

   Article

   Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

   Insert

   COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ND6-L1397-N_UGI-NES_left

   Plasmid

   #187845

   Purpose

   expresses the left-side half ND6-UGI-NES in mammalian cells

   Depositor

   Chengqi Yi

   Article

   Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

   Insert

   SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS416-PTEF1-MCP-no_intron

   Plasmid

   #127597

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   1xFLAG-MCP expression cassette
   

   Expression

   Yeast

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a(+)/FbGH17B

   Plasmid

   #100912

   Purpose

   Expression of the Beta-1,3-Exo-Laminarinase FbGH17B in E.coli

   Depositor

   Jan-Hendrik Hehemann

   Insert

   FbGH17Bcat
   

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   not full length

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK-EdnrbE1-Citrine

   Plasmid

   #127773

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE1
   

   Use

   Enhancer reporter construct

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET3a-hUBA1

   Plasmid

   #63571

   Purpose

   Expression of human UBA1 tagged with His

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBA1 (UBA1 Human)
   

   Expression

   Bacterial

   Mutation

   Isoform 2 (1018 amino acids)

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

   Plasmid

   #229498

   Purpose

   This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   MYC 5'Untranslated region + destabilized GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. mScarletI3-6DG5-MAPPER

   Plasmid

   #222421

   Purpose

   To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   MAPPER
   

   Tags

   mScarletI3-6DG5

   Expression

   Mammalian

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS117 His14-Avi-45xGS-anti GFP nanobody

    Plasmid

    #199370

    Purpose

    Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-GFP nanobody
    

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmScarlet3_C1

    Plasmid

    #189753

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CamKII.GCaMP6s.WPRE.SV40

    Plasmid

    #107790

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-GAL9

    Plasmid

    #166689

    Purpose

    For creating a stable mCherry-GAL9 cell line

    Depositor

    Alan Sabirsh

    Article

    Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

    Insert

    LGALS9 (LGALS9 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p53-GFP

    Plasmid

    #11770

    Depositor

    Geoff Wahl

    Insert

    p53 (TP53 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    72R variant of p53

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. SBE4-Luc

    Plasmid

    #16495

    Purpose

    Luciferase reporter containing four copies of the Smad binding site

    Depositor

    Bert Vogelstein

    Article

    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

    Insert

    Smad binding element
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV.CBA.eGFP.2A.P301L-Tau

    Plasmid

    #140425

    Purpose

    AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
    

    Use

    AAV

    Tags

    eGFP

    Mutation

    changed Proline 301 to Leucine

    Promoter

    CBA

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mscv2.2-NLRC4

    Plasmid

    #60199

    Purpose

    Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PtipA; crRNA under control of PermE*

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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