165,965 results

1. pARC8-LambdaRedBeta-EcSSB

   Plasmid

   #162572

   Purpose

   Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

   Depositor

   George Church

   Article

   Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

   Inserts

   Lambda Red-Beta
   E. coli SSB
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   May 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-C 16E7

   Plasmid

   #37880

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPV 16 E7 (E7 HPV)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pemiRFP670-N1

   Plasmid

   #136556

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP670
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. dRED

   Plasmid

   #139836

   Purpose

   Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Expression

   Mammalian

   Mutation

   RNase H1 protein contains D210N mutation. dCas9 …

   Promoter

   tet-inducible CMV

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2-hspDRv7_scGstlt

   Plasmid

   #108870

   Purpose

   scGESTALT V7 lineage barcode for zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Insert

   scGESTALT lineage tracing target V7
   

   Use

   Tol2 integration into zebrafish genome

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-sgAAVS1-2

   Plasmid

   #129727

   Purpose

   Expressing SpCas9 and sgAAVS1-2

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-2
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST51-LRRK2-R1441C

   Plasmid

   #25081

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Destination vector

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Arginine 1441 mutated to Cysteine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pUBC-SV40NLS-Pfv-Sapphire-PURO

   Plasmid

   #203654

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEmerald-CLIP170-C-18

    Plasmid

    #54043

    Purpose

    Localization: Microtubule Binding , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CLIP170 (CLIP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pMIN-VS1

    Plasmid

    #197783

    Purpose

    Level 2 destination vector: pMIN-VS1 backbone

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIPZ-shNTC

    Plasmid

    #217638

    Purpose

    Dox inducible shRNA non targeting control shRNA

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    shNTC
    

    Use

    RNAi

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-cys-His6-OtUBD

    Plasmid

    #190091

    Purpose

    Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4-AARE-luc2P-Hygro

    Plasmid

    #101787

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    3xAARE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 puro_shTBK1_3185

    Plasmid

    #167294

    Purpose

    Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    shRNA TBK1 (TBK1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-hSyn1-GFP

    Plasmid

    #177810

    Purpose

    Encodes a GFP driven by human synapsin I promoter

    Depositor

    Christopher Grigsby

    Article

    Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

    Insert

    CopGFP inserted behind human synapsin I promoter (SYN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Syn1

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-TetOminiCMV-SV40LT

    Plasmid

    #136616

    Purpose

    Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    SV40 large T antigen
    

    Use

    Lentiviral

    Expression

    Bacterial

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHM129

    Plasmid

    #225534

    Purpose

    Encodes destablized mCherry under AR8 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    mCherry
    BFP
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-SRRM2-mCherry

    Plasmid

    #174089

    Purpose

    Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

    Depositor

    Wesley Sundquist, Katie Ullman

    Article

    Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

    Insert

    SRRM2 (SRRM2 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Sept. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLDLR-Luc mutSRE

    Plasmid

    #14945

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter mut SRE (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Point mutation in the SRE-1 sequence of the LDL r…

    Available Since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid

    #172507

    Purpose

    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pCMV - huDisCas7-11 mammalian expression
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV5-CBFbeta

    Plasmid

    #12427

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    CBF beta (Cbfb Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pAAV-CMVie-NcanGFP

    Plasmid

    #235983

    Purpose

    Expresses the hyaluronan-binding domain of Neurocan fused to eGFP under the CMV promoter. It contains a sequence for secretory pathway, so the probe is exported to the extracellular space

    Depositor

    Federico Soria

    Article

    Fernández-Ballester et al bioRxiv 2025.01.31.635882; doi: 10.1101/

    Insert

    Neurocan-GFP
    

    Use

    AAV

    Tags

    GFP

    Promoter

    CMVie

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pPROBE-NT

    Plasmid

    #37818

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FLAG-3C-sGFP11_pcDNA3.1(-)

    Plasmid

    #182932

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Tags

    3C-cleavable split GFP 11, FLAG tag, and HA signa…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLenti6.2_TagBFP

    Plasmid

    #113724

    Purpose

    Expresses the fluorescent protein TagBFP in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid

    #135281

    Purpose

    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-CDK12 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSNAP-CaaX Control Plasmid

    Plasmid

    #101133

    Purpose

    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    CaaX Box
    

    Tags

    SNAP-tag (SNAP26m)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. Butelase AY

    Plasmid

    #221847

    Purpose

    Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

    Insert

    Butelase 1 ligase
    

    Expression

    Bacterial

    Mutation

    Val237Ala, Thr238Tyr

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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