12,913 results

Showing: 3451 - 3480 of 12913 results

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Lenti-sgNeo/Cre

Plasmid

#67594

Purpose

Expresses an Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgNeo

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-HA-ERK5-FL

Plasmid

#31817

Depositor

Astar Winoto

Article

Kasler et al Mol Cell Biol. 2000 Nov;20(22):8382-9.

Insert

Erk5 (Mapk7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Hes1

Plasmid

#61534

Purpose

Expresses Hes1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes1 (Hes1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Gfi1b

Plasmid

#131611

Purpose

expression of mouse Gfi1b in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Gfi1b (Gfi1b Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS mSynCAM1

Plasmid

#115496

Purpose

expression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm1 (Cadm1 Mouse)

Expression

Mammalian

Promoter

chicken beta-actin

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only
pCMV-PE2-p53DD

Plasmid

#196582

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Expression

Mammalian

Mutation

Δ40-903

Promoter

CMV

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pB3.P11

Plasmid

#69577

Purpose

OT-I TCR beta chain cDNA in pCRScript

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR beta cDNA clone B3 (Tcrb Mouse)

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag-SIRT1 H355A

Plasmid

#8439

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap1g1-FKBP

Plasmid

#46945

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap1g1-FKBP

Tags

Mainly mouse Ap1g1 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pM2AH + mSlo1

Plasmid

#16152

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)

Expression

Mammalian

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pMX-mTAF6

Plasmid

#44337

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-C2-SNAP

Plasmid

#208794

Purpose

For AAV production to express C2-SNAP in astrocytes

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2-SNAP (Mfge8 Mouse)

Use

AAV

Tags

SNAP-tag

Expression

Mammalian

Promoter

GFAP promoter

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-C2m2-SNAP

Plasmid

#208791

Purpose

For AAV production to express C2m2-SNAP in astrocytes

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Use

AAV

Tags

SNAP-tag

Expression

Mammalian

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

GFAP promoter

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-KM

Plasmid

#20332

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMX-mTAF13

Plasmid

#44343

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF13 (Taf13 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ME-Flag

Plasmid

#101860

Purpose

Mds1-Evi-1 flag tagged for transfection into mammalian cells

Depositor

Archibald Perkins

Insert

Mds1-Evi-1 (Mecom Mouse)

Tags

Flag

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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myc-mULK1M92A

Plasmid

#31962

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Tags

myc

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available Since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
pMX-mTAF9

Plasmid

#44339

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF9 (Taf9 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pKT-mPDGFb-IRES-Katushka

Plasmid

#133082

Purpose

Expresses mouse PDGFb and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Insert

PDGFb (Pdgfb Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-mFgf21pro2-luc

Plasmid

#101798

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53 Δ1-42

Plasmid

#196269

Purpose

Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 Δ1-42 (Trp53 Mouse)

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 1 to 42

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-NeuroD2iresGFP

Plasmid

#45026

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD2 (Neurod2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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PDGFRalpha-D842V #1

Plasmid

#201407

Purpose

Inserts PDGFRalpha-D842V to activate the MAP kinase pathway.

Depositor

Maria Castro

Article

Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

Insert

PDGFRalpha-D842V (Pdgfra Mouse)

Expression

Mammalian

Mutation

D842V

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Sema6b(L)-AP-His

Plasmid

#72039

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Notch2_B9-pBIOCAM5

Plasmid

#39348

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Mouse, Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700-mouse-ACTC1-Puro

Plasmid

#128326

Purpose

SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

Depositor

Alejandro Chavez

Insert

gRNA against mouse ACTC1 for activation (Actc1 Mouse)

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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12,913 results