We narrowed to 8,683 results for: sgRNA

1. pJMP8932

   Plasmid

   #220906

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. hLig4 guide2 in pX458

   Plasmid

   #211536

   Purpose

   sgRNA-2 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hLig4 guide1 in pX458

   Plasmid

   #211535

   Purpose

   sgRNA-1 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-TC9

   Plasmid

   #202088

   Purpose

   entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

   Depositor

   David Edgell

   Article

   Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

   Insert

   TevCas9 + sgRNA cassette
   

   Use

   CRISPR

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p8271 LentiCRISPR v2 hygro sgSIGIRR-4

   Plasmid

   #193980

   Purpose

   Expression of spCas9 and sgRNA targeting SIGIRR

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT-GFP-rG1

   Plasmid

   #188967

   Purpose

   IPTG inducible GFP with sgRNA

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   GFP
   sgRNA: agtggaaaacaatgcgaccgactagt
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptrc

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT-GFP-rG2

   Plasmid

   #188968

   Purpose

   IPTG inducible GFP with sgRNA

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   GFP
   sgRNA: agtccatgtaatcagcgtctactagt
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptrc

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PHY55-mouse U6-sgLMNA

   Plasmid

   #164045

   Purpose

   U6-driven sgRNA targeting LMNA

   Depositor

   Baohui Chen

   Article

   Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

   Insert

   LMNA targeting sgRNA
   

   Use

   CRISPR

   Promoter

   mouse U6

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJF449_dpy-10_CDS_sg1

   Plasmid

   #163866

   Purpose

   sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting the coding sequence of dpy-10
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   R07E5.16 (U6)

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJF328_sqt-3_3′UTR_sg2

    Plasmid

    #163867

    Purpose

    sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the 3'UTR of sqt-3
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    R07E5.16 (U6)

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJJF496_dpy-10_CDS_sg6

    Plasmid

    #164267

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    R07E5.16 (U6)

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJF495_dpy-10_CDS_sg6

    Plasmid

    #164268

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6 promoter from W05B2.8

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGuide-P1-O1a (EL702)

    Plasmid

    #140040

    Purpose

    CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

    Depositor

    Johan Elf

    Article

    Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

    Insert

    barcode P1 and sgRNA lacO1 array
    

    Use

    Synthetic Biology

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-hsg(NT:NT)

    Plasmid

    #138260

    Purpose

    Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgPYM1

    Plasmid

    #105249

    Purpose

    human PYM1 sgRNA

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    sgRNA for PYM1
    

    Expression

    Mammalian

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209060

    Purpose

    Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
    

    Use

    Gateway vector to be used for lr reaction

    Promoter

    U6

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXPR_045

    Plasmid

    #107144

    Purpose

    cloning of a sgRNA

    Depositor

    David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLib6.6

    Plasmid

    #176652

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEditA

    Plasmid

    #207531

    Purpose

    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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