We narrowed to 6,336 results for: WUS;

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pGEX-5X-TRIM28

Plasmid

#45570

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Tags

GST

Expression

Bacterial

Promoter

Ptac

Available Since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-oB ONE-GO

Plasmid

#189733

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oB ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28

Plasmid

#223596

Purpose

Lentiviral expression of human CD28 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i2 ONE-GO

Plasmid

#189736

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i2 ONE-GO
GNAI2 (Gnai2 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

Plasmid

#172985

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FAB(ER-mito)

Plasmid

#214427

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-N-HA-Pcdh15_mini_V4

Plasmid

#199185

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

HA-mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Human influenza hemagglutinin (HA)

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-5

Plasmid

#228965

Purpose

For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 S156A

Plasmid

#127588

Purpose

Phospho-mutant of S156

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S156A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Th-iCre

Plasmid

#159459

Purpose

Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

P2A-iCre

Use

Cre/Lox

Tags

P2A

Expression

Mammalian

Promoter

Endogenous TH

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pM-TagRFP657

Plasmid

#176563

Purpose

Expression of TagRFP657 for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

TagRFP657

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

Plasmid

#187579

Purpose

Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

PSPC1 (PSPC1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(mito-LD)

Plasmid

#214421

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-3XHA-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB304_ZFcharm_Kv1_Prnp_SCR

Plasmid

#220842

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

Plasmid

#240799

Purpose

CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

mNeonGreen-mCherry-CAAX

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-mNeon-Stop-PGK-mCherry-CAAX

Plasmid

#240800

Purpose

CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via PGK promoter

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

mNeonGreen mCherry-CAAX

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:KIT K642E

Plasmid

#118849

Purpose

Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT K642E (KIT Zebrafish, Human)

Use

Zebrafish expression, tol5

Mutation

KIT K642E

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-14

Plasmid

#228994

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet1 CD Mut

Plasmid

#72218

Purpose

Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain (cata…

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pmRFP-N1 human cofilin S3A

Plasmid

#50857

Purpose

expresses constituvely active cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCDH-CMV-RPS15A-3xHA plasmid

Plasmid

#192245

Purpose

overexpression vector of RPS15A-3XHA

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

RPS15A CDS without stop coden (RPS15A Human)

Use

Lentiviral

Tags

3xHA

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL1_P2A_Hygro_Barcode

Plasmid

#120427

Purpose

Barcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-mCherry

Plasmid

#159458

Purpose

AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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VPS13B^GFP

Plasmid

#224588

Purpose

codon optimized GFP tagged VPS13B for expression in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

VPS13B^GFP (VPS13B Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCA12-pHR-SFFV-BFP-B23

Plasmid

#199443

Purpose

Expression of BFP-B23 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

B23 (NPM1 Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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