159,333 results

1. pcDNA3.1-AXL-V5/HIS

   Plasmid

   #200117

   Purpose

   Expression of human AXL receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human AXL receptor tyrosine kinase (AXL Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyso-ExRai-AMPKAR

   Plasmid

   #192449

   Purpose

   ExRai-AMPKAR targeted to the lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   Lyso-ExRai-AMPKAR
   

   Use

   Tags

   Full-length LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCap-101-Hgy

   Plasmid

   #227508

   Purpose

   Capture plasmid with hygromycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcs2-GFP-IRES-mCherry

   Plasmid

   #226098

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_479

   Plasmid

   #179099

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   ABE8e (SpG)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. JunB

   Plasmid

   #187903

   Purpose

   Expresses JunB in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   JunB (Junb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CT3G-CERP2-MG

   Plasmid

   #175503

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-sgR26

    Plasmid

    #127376

    Purpose

    Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBiex-1 GFPNanobody_SNAP

    Plasmid

    #82711

    Purpose

    Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    GFP Nanobody
    

    Use

    Tags

    6xHis Purification tag, FLAG purification tag, an…

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MI-Luciferase-IRES-mCherry

    Plasmid

    #75020

    Purpose

    Encodes the expression of Luciferase and mCherry

    Depositor

    Xiaoping Sun

    Article

    Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

    Inserts

    firefly Luciferase
    mCherry
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-blast-SCRAMBLE

    Plasmid

    #26701

    Purpose

    3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CAG-GFP

    Plasmid

    #28014

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Insert

    eGFP
    

    Use

    AAV; Adeno associate viral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid

    #58477

    Purpose

    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Arginine 62 to Aspartic Acid

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tet-pLKO-neo

    Plasmid

    #21916

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Use

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid

    #26730

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p006-GFP-strong

    Plasmid

    #108313

    Purpose

    GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4026

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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